RECOMMENDED README FILE FOR GREDOS_USAL This readme.txt file was generated on YYYYMMDD by Name ---------------------------------- GENERAL INFORMATION ---------------------------------- 1. Title of Dataset: Multisession anodal epidural direct current stimulation of the auditory cortex delays the progression of presbycusis in the Wistar rat 2. Authors: ✓ Name: Merchán Cifuentes, Miguel Ángel Alvarado, Juan Carlos Plaza Lopez, Ignacio Díaz, Iván Santos Fernández del Campo, Inés Carmona Barrón, Venezia Guadalupe ✓ Institution:USAL-INCYL ✓ Email:merchan@usal.es ✓ ORCID:0000-0002-1334-2822 ------------------- DESCRIPTION ------------------- 1. Dataset language:English 2. Abstract:Presbycusis or age-related hearing loss (ARHL) is one of the most prevalent chronic health problems facing aging populations. Along the auditory pathway, the stations involved in transmission and processing, function as a system of interconnected feedback loops. Regulating hierarchically auditory processing, auditory cortex (AC) neuromodulation can, accordingly, activate both peripheral and central plasticity after hearing loss. However, previous ARHL-prevention interventions have mainly focused on preserving the structural and functional integrity of the inner ear, overlooking the central auditory system. In this study, using an animal model of spontaneous ARHL, we aim at assessing the effects of multisession epidural direct current stimulation of the AC through stereotaxic implantation of a 1-mm silver ball anode in Wistar rats. Consisting of 7 sessions (0.1 mA/10 min), on alternate days, in awake animals, our stimulation protocol was applied at the onset of hearing loss (threshold shift detection at 16 months). Click- and pure-tone auditory brainstem responses (ABRs) were analyzed in two animal groups, namely electrically stimulated (ES) and non-stimulated (NES) sham controls, comparing recordings at 18 months of age. At 18 months, NES animals showed significantly increased threshold shifts, decreased wave amplitudes, and increased wave latencies after click and tonal ABRs, reflecting a significant, spontaneous ARHL evolution. Conversely, in ES animals, no significant differences were detected in any of these parameters when comparing 16 and 18 months ABRs, indicating a delay in ARHL progression. Electrode placement in the auditory cortex was accurate, and the stimulation did not cause significant damage, as shown by the limited presence of superficial reactive microglial cells after IBA1 immunostaining. In conclusion, multisession DC stimulation of the AC has a protective effect on auditory function, delaying the progression of presbycus 3. Keywords: Age-related hearing loss Aging Auditory brainstem responses IBA1 Medial olivo-cochlear efferent system Tonotopy 4. Date of data collection:2024-03-15 5. Date of data publication on repository:28/11/2025 6. Funding (Information about funding sources that supported the collection of the data):This study was funded by the Spanish Ministry of Science and Innovation (Ministerio de Ciencia e Innovación – MICINN) , through grant PID2020-117266RB-C21 awarded to M. Merchan and J.M. Juiz. 7. Geographic location of data collection : 8. Recommended citation for this dataset: Fernández Del Campo, I. S., Carmona-Barrón, V. G., Diaz, I., Plaza, I., Alvarado, J. C., y Merchán, M. A. (2024). Multisession anodal epidural direct current stimulation of the auditory cortex delays the progression of presbycusis in the Wistar rat. Hearing Research, 444, 108969. https://doi.org/10.1016/j.heares.2024.108969 --------------------------------------------------------- SHARING/ACCESS/CONTEXT INFORMATION --------------------------------------------------------- 1. Usage Licenses/restrictions placed on the data (please indicate if different data files have different usage license). (Creative commons 4.0- BY-NC-ND o similar): CC BY-NC 4.0 2. Related publications: 3. Dataset DOI: -------------------------------- DATA & FILE OVERVIEW -------------------------------- 1. File List: Copia de Figure 1 .tif Copia de Figure 2 .tif Copia de Figure 3.tif Copia de Figure 4.tif Copia de Figure 5.tif Nombre:Copia de Figure 6.tif Copia de Figure 7.tif Copia de Figure 8.tif Copia de Figure 9 Supp Mat.tif 2. Relationship between files, if important: 3. File format:.tif ----------------------------------------------- METHODOLOGICAL INFORMATION ---------------------------------------------- 1. Instrument- or software-specific information needed to interpret/reproduce the data, please indicate their location: 2. Describe any quality-assurance procedures performed on the data: 3. Author contact information: merchan@usal.es