RECOMMENDED README FILE FOR GREDOS_USAL

This readme.txt file was generated on YYYYMMDD by Name

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GENERAL INFORMATION
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1. Title of Dataset:
Comparing the effects of transcranial alternating current and temporal interference (tTIS) electric stimulation through whole-brain mapping of c-Fos immunoreactivity
2. Authors:
✓ Name:Carmona Barrón, Venezia Guadalupe
Fernández del Campo, Inés S.
Delgado-Garcia, Jose Maria
Fuente Juan, Antonio de la
Plaza Lopez, Ignacio
Merchán Cifuentes, Miguel Ángel
✓ Institution:INCYL-USAL
✓ Email:merchan@usal.es
✓ ORCID:0000-0002-1334-2822
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DESCRIPTION
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1. Dataset language:English
2. Abstract:
The analysis of the topography of brain neuromodulation following transcranial alternating current (AC) stimulation is relevant for defining strategies directed to specific nuclei stimulation in patients. Among the different procedures of AC stimulation, temporal interference (tTIS) is a novel method for non-invasive neuromodulation of specific deep brain targets. However, little information is currently available about its tissue effects and its activation topography in in vivo animal models. After a single session (30 min, 0.12 mA) of transcranial alternate current (2,000 Hz; ES/AC group) or tTIS (2,000/2,010 Hz; Es/tTIS group) stimulation, rat brains were explored by whole-brain mapping analysis of c-Fos immunostained serial sections. For this analysis, we used two mapping methods, namely density-to-color processed channels (independent component analysis (ICA) and graphical representation (MATLAB) of morphometrical and densitometrical values obtained by density threshold segmentation. In addition, to assess tissue effects, alternate serial sections were stained for glial fibrillary acidic protein (GFAP), ionized calcium-binding adapter molecule 1 (Iba1), and Nissl. AC stimulation induced a mild superficial increase in c-Fos immunoreactivity. However, tTIS stimulation globally decreased the number of c-Fos-positive neurons and increased blood brain barrier cell immunoreactivity. tTIS also had a stronger effect around the electrode placement area and preserved neuronal activation better in restricted areas of the deep brain (directional stimulation). The enhanced activation of intramural blood vessels’ cells and perivascular astrocytes suggests that low-frequency interference (10 Hz) may also have a trophic effect
3. Keywords:
blood brain barrier (BBB)
deep brain simulation
GFAP - glial fibrillary acidic protein
IBA1 - ionized calcium binding adaptor molecule 1
independent component analysis (ICA transform)
rat - brain
4. Date of data collection:2023-03-13
5. Date of data publication on repository:28/11/2025
6. Funding (Information about funding sources that supported the collection of the data):This study was funded by the Spanish Ministry of Science and Innovation (Ministerio de Ciencia e Innovación—MICINN), through grant PID2020-117266RB-C21 awarded to MM and through PID201-122446NB-100 awarded to JD-G.
7. Geographic location of data collection <latitude, longitude, or city/region, Country, continent
as appropriate>:Salamanca, Spain
8. Recommended citation for this dataset:Del Campo, I. S. F., Carmona-Barrón, V. G., Diaz, I., Plaza, I., Alvarado, J., & Merchán, M. (2024). Multisession anodal epidural direct current stimulation of the auditory cortex delays the progression of presbycusis in the Wistar rat. Hearing Research, 444, 108969. https://doi.org/10.1016/j.heares.2024.108969
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SHARING/ACCESS/CONTEXT INFORMATION
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1. Usage Licenses/restrictions placed on the data (please indicate if different data files have
different usage license).
 (Creative commons 4.0- BY-NC-ND o similar): Attribution-NonCommercial-NoDerivatives 4.0 International
2. Related publications:
3. Dataset DOI:
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DATA & FILE OVERVIEW
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1. File List:Copia de Carmona et al sec ve.pdf Copia de Fig 1 Carmona et al new.tif Copia de Fig 2TIF.TIF Copia de FIg 3 Carmona et al new .tif Copia de Fig 5.tif Copia de Fig 6 08 feb.tif Copia de Fig 7 Carmona et al new.tif Copia de Fig 8 Carmona et al new.tif Copia de Fig 9 Carmona et al new.tif
2. Relationship between files, if important:
3. File format:.tif
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METHODOLOGICAL INFORMATION
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1. Instrument- or software-specific information needed to interpret/reproduce the data, please
indicate their location: 
2. Describe any quality-assurance procedures performed on the data:
3. Author contact information: merchan@usal.es