RECOMMENDED README FILE FOR GREDOS_USAL

This readme.txt file was generated on YYYYMMDD by Name

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GENERAL INFORMATION
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1. Title of Dataset:
Anodal direct current stimulation of the auditory cortex at the onset of presbycusis delays cortical aging
2. Authors:
✓ Name:Merchán Cifuentes, Miguel Ángel
Plaza Lopez, Ignacio
Díaz, Iván
Fuente Juan, Antonio de la
Santos Fernández del Campo, Inés
✓ Institution:INCYL-USAL
✓ Email: merchan@usal.es
✓ ORCID:0000-0002-1334-2822
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DESCRIPTION
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1. Dataset language: English
2. Abstract:
Presbycusis or age-related hearing loss (ARHL) affects millions of people worldwide, increasing their risk of cognitive decline and poor quality of life. However, ARHL remains an irreversible condition due to our inability to induce inner-ear hair cell regeneration. Nevertheless, multisession epidural stimulation of the auditory cortex (AC) at the onset of ARHL prevents hearing threshold elevation in naturally aging Wistar rats. Accordingly, we hypothesized that anodal direct current (DC) stimulation of the AC may also compensate for age-related maladaptive, activity-dependent changes. Here, we examined immunocytochemical markers in the AC, including early genes (c-fos and Arc), AMPA receptors (GluR2/3), parvalbumin (PV), and GAD67, along with auditory-evoked potentials (CAEPs) recorded in both auditory and visual (VC) cortices. When comparing 6 and 18.13-month-old rats without AC simulation, we observed loss of c-fos and Arc-positive neurons and decreased GluR2/3 expression, confirming altered AC neuronal network plasticity and activation. In addition, we noted changes in PV and decreased GAD67 immunoreactivity suggesting disrupted inhibition and significantly increased wave amplitudes in CAEPs, altered AC latencies, and decreased VC responses. By contrast, electrically stimulated rats showed no significant variations in early gene markers, GluR2/3, PV, or GAD67 with age, and the amplitudes and latencies of CAEPs recorded in their AC and VC resembled those of young rat. These findings indicate that anodal DC stimulation at the onset of ARHL delays AC aging by minimizing the loss of inhibition and preventing increases in cortical excitability in Wistar rats
3. Keywords:
Age related hearing loss
Cortical excitability
Excitatory/inhibitory balance
Natural aging
Wistar rat
4. Date of data collection:2025-04-25
5. Date of data publication on repository:28/11/2025
6. Funding (Information about funding sources that supported the collection of the data):Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. Junta de Castilla y Le\u00F3n,Ministerio de Ciencia e Innovaci\u00F3n,PID2020-117266RB-C21,PID2020-117266RB-C21,PID2020-117266RB-C21,PID2020-117266RB-C21
7. Geographic location of data collection <latitude, longitude, or city/region, Country, continent
as appropriate>:Salamanca, Spain
8. Recommended citation for this dataset:Fernández Del Campo, I. S., De La Fuente, A. J., Díaz, I., Plaza, I., y Merchán, M. A. (2025). Anodal direct current stimulation of the auditory cortex at the onset of presbycusis delays cortical aging. Brain Structure and Function, 230(4), 56. https://doi.org/10.1007/s00429-025-02912-w
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SHARING/ACCESS/CONTEXT INFORMATION
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1. Usage Licenses/restrictions placed on the data (please indicate if different data files have
different usage license).
 (Creative commons 4.0- BY-NC-ND o similar): Attribution-NonCommercial 4.0 International
2. Related publications:
3. Dataset DOI:
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DATA & FILE OVERVIEW
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1. File List:Copia de Fig 1 Fernandez del Campo et al.,.tif Copia de Fig 2 Fernandez del Campo et al.,.tif Copia de Fig 3 Fernandez del Campo et al.,.tif Copia de Fig 4 Fernandez del Campo et al.,.tif Copia de Fig 5 Fernandez del Campo et al.,.tif Copia de Fig 6 Fernandez del Campo et al.,.tif Copia de Fig 7 Fernandez del Campo et al.,.tif Copia de Fig 8 Fernandez del Campo et al.,.tif Copia de Fig 9 Fernandez del Campo et al.,.tif Copia de Fig 10 Fernandez del Campo et al.,.tif Copia de Fig 11 Fernandez del Campo et al.,.tif Copia de Supp Mat Fernandez del Campo et al.,.tif 
2. Relationship between files, if important:
3. File format:.tif
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METHODOLOGICAL INFORMATION
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1. Instrument- or software-specific information needed to interpret/reproduce the data, please
indicate their location: 
2. Describe any quality-assurance procedures performed on the data:
3. Author contact information: merchan@usal.es