http://hdl.handle.net/10366/156461 Fri, 01 May 2026 05:57:50 GMT 2026-05-01T05:57:50Z http://hdl.handle.net/10366/166999 Arabinogalactan proteins (AGPs) are involved in various physiological processes, such as cell elongation, xylem differentiation, resistance to abiotic stresses or secretion and adherence of seed coat mucilage, a structure suggested as a model system for cell wall studies. The specific roles of AGPs are not fully established, although their carbohydrate motif (type II arabinogalactan, AGII) seems to be essential, being able to mediate interactions with different signalling molecules or with other cell wall polysaccharides. The aim of the present work is to determine the role of AGII from AGPs in the structural organization of the cell wall, using Arabidopsis thaliana plants that overproduce β-galactosidase βV-Gal from Cicer arietinum (35S::βV-Gal plants), an enzyme that acts specifically on the β-(1,3) and β-(1,6)-galactosyl bonds of AGII. The characterization of the seed coat mucilage has allowed us to establish a cell wall homeostasis mechanism in which the neutral side chains of the AGII of the AGPs determine the degree of HG methyl esterification. Thus, the reduction in the galactose is accompanied by an increase in the level of esterification, probably as a compensatory mechanism to maintain the mechanical properties of this specialized cell wall and its hydration properties. Wed, 01 Jan 2025 00:00:00 GMT http://hdl.handle.net/10366/166999 2025-01-01T00:00:00Z http://hdl.handle.net/10366/166998 [EN] Proteins of the PF10950 family feature the DUF2775 domain of unknown function. The most studied are specific tissue (ST) proteins with tandem repeats, which are putative precursors of cyclopeptide alkaloids. Here, we study uncharacterised short ST (SST) proteins with the DUFF2775 domain by analysing 194 sequences from 120 species of 39 taxonomic families in silico. SST proteins have a signal peptide and their size and several other characteristics depend on their individual taxonomic family. Sequence analyses revealed that SST proteins contain two well-conserved regions, one resembling the ST repeat, which could constitute the core of cyclopeptide alkaloids. We studied the unique SST1 gene of Arabidopsis thaliana, which is adjacent to and co-expressed with a gene encoding a protein with a BURP domain, associated with cyclopeptide production. The empirical analysis indicated that the SST1 promoter is mainly activated in the roots, where most of the transcripts accumulate, and that the SST1 protein accumulates in the root vascular cambium. At the cellular level, SST fused to GFP appears in vesicles that colocalise with the endoplasmic reticulum and the vacuole. Thus, SSTs are a new type of PF10950 protein found in core eudicots with two conserved regions that could be involved in root biology. Tue, 01 Apr 2025 00:00:00 GMT http://hdl.handle.net/10366/166998 2025-04-01T00:00:00Z http://hdl.handle.net/10366/159764 Mon, 01 Jan 2024 00:00:00 GMT http://hdl.handle.net/10366/159764 2024-01-01T00:00:00Z http://hdl.handle.net/10366/157822 Sun, 01 Jan 2006 00:00:00 GMT http://hdl.handle.net/10366/157822 2006-01-01T00:00:00Z http://hdl.handle.net/10366/157821 En este artículo se pretende de forma muy general explicar qué es una planta transgénica, cómo se construye, es decir, que pasos hay que dar hasta obtenerla y qué aplicaciones tienen. Veremos, entre otras, aplicaciones agrícolas, como la producción de plantas resistentes a herbicidas, y aplicaciones industriales como la producción de vacunas u otros compuestos de interés terapéutico. Finalmente analizaremos alguno de los aspectos polémicos y el rechazo que la construcción de plantas transgénicas ha generado. Thu, 01 Jan 2004 00:00:00 GMT http://hdl.handle.net/10366/157821 2004-01-01T00:00:00Z http://hdl.handle.net/10366/157820 Pectin is a collection of polysaccharides, which play an important role in controlling the pore size of the plant cell wall, regulating cell-cell adhesion, and providing a source of signalling molecules that elicit a range of cellular responses. Apart from this, pectins are of interest because they are an attractive hydrocolloid for various food applications. The kind and distribution of decorative groups in the pectic molecules largely determines for which application a particular pectin is most suitable. After the extraction of starch from potato tubers, a by-product is obtained, which is relatively rich in pectin. However, the quality of these pectins is poor compared to that from other sources such as citrus and apple. Rather than trying to change the structural cha.racteristics of potato pectin post-harvest, we have embarked on achieving this in the potato plant itself. This paper summarises the structural features of pectin, the distribution of various peetic epitopes in tuber cell walls, the enzymes involved in its biosynthesis and degradation, and strategies employed to alter its fine structure in planta. Sat, 01 Jan 2000 00:00:00 GMT http://hdl.handle.net/10366/157820 2000-01-01T00:00:00Z http://hdl.handle.net/10366/157819 Tue, 01 Jan 1991 00:00:00 GMT http://hdl.handle.net/10366/157819 1991-01-01T00:00:00Z http://hdl.handle.net/10366/157631 The Electronic Plant Gene Register Fri, 01 Jan 1999 00:00:00 GMT http://hdl.handle.net/10366/157631 1999-01-01T00:00:00Z http://hdl.handle.net/10366/157630 The Electronic Plant Gene Register Fri, 01 Jan 1999 00:00:00 GMT http://hdl.handle.net/10366/157630 1999-01-01T00:00:00Z http://hdl.handle.net/10366/157629 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157629 1998-01-01T00:00:00Z http://hdl.handle.net/10366/157628 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157628 1998-01-01T00:00:00Z http://hdl.handle.net/10366/157627 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157627 1998-01-01T00:00:00Z http://hdl.handle.net/10366/157626 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157626 1998-01-01T00:00:00Z http://hdl.handle.net/10366/157625 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157625 1998-01-01T00:00:00Z http://hdl.handle.net/10366/157624 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157624 1998-01-01T00:00:00Z http://hdl.handle.net/10366/157623 The Electronic Plant Gene Register Thu, 01 Jan 1998 00:00:00 GMT http://hdl.handle.net/10366/157623 1998-01-01T00:00:00Z