http://hdl.handle.net/10366/4191 Wed, 06 May 2026 10:33:48 GMT 2026-05-06T10:33:48Z http://hdl.handle.net/10366/145069 [EN] We confirmed the ability of the triterpenoid betulin to protect against neurotoxicity caused by Bothrops jararacussu snake venom in vitro in mouse isolated phrenic nerve-diaphragm (PND) preparations and examined its capability of in vivo protection using the rat external popliteal/sciatic nerve-tibialis anterior (EPSTA) preparation. Venom caused complete, irreversible blockade in PND (40 𝜇g/mL), but only partial blockade (∼30%) in EPSTA (3.6 mg/kg, i.m.) after 120 min. In PND, preincubation of venom with commercial bothropic antivenom (CBA) attenuated the venom-induced blockade, and, in EPSTA, CBA given i.v. 15 min after venom also attenuated the blockade (by ∼70% in both preparations). Preincubation of venom with betulin (200 𝜇g/mL) markedly attenuated the venom-induced blockade in PND; similarly, a single dose of betulin (20 mg, i.p., 15 min after venom) virtually abolished the venom-induced decrease in contractility. Plasma creatine kinase activity was significantly elevated 120 min after venom injection in the EPSTA but was attenuated by CBA and betulin. These results indicate that betulin given i.p. has a similar efficacy as CBA given i.v. in attenuating the neuromuscular effects of B. jararacussu venom in vivo and could be a useful complementary measure to antivenom therapy for treating snakebite. Thu, 01 Jan 2015 00:00:00 GMT http://hdl.handle.net/10366/145069 2015-01-01T00:00:00Z http://hdl.handle.net/10366/22600 Los compuestos químicas presentes en plantas y animales siguen siendo una fuente importante de fármacos y de productos usados en la alimentación, la cosmética y laagricultura entre otros campos, o bien como fuente para la obtención de principios activos de interés biológico y farmacológico. Mon, 01 Dec 2008 00:00:00 GMT http://hdl.handle.net/10366/22600 2008-12-01T00:00:00Z