Nitric oxide synthase activity in the olfactory bulb of anuran and urodele amphibians
Nitric oxide synthase
Fecha de publicación
Porteros A, Arévalo R, Crespo C, Briñón JG, Weruaga E, Aijón J, Alonso (1996). Nitric oxide synthase activity in the olfactory bulb of anuran and urodele amphibians. JR. Brain Res. 1996Jun 10;724(1):67-72
Nitric oxide synthase activity was studied by means of NADPH-diaphorase activity and nitric oxide synthase immunoreactivity in the main and accessory olfactory bulbs of the frog Rana perezi and the newt Triturus marmoratus. In both species, NADPH-diaphorase staining was observed in all olfactory fibers. Vomeronasal fibers were NADPH-diaphorase labeled in Triturus but they were NADPH-diaphorase negative in Rana. Nitric oxide synthase immunoreactivity was not observed in the primary afferents in any case.Granule cells were NADPH-diaphorase positive and nitric oxide synthase immunopositive in the main and accessory olfactory bulb of Rana, and in the main olfactory bulb of Triturus. The homogeneous NADPH-diaphorase staining of olfactory fibers is similar to what has been reported in teleosts, and it contrasts with the spatial segregation of NADPH-diaphorase positive and negative olfactory projections in rodents. These results confirm the interspecies variability of the NADPH-diaphorase/nitric oxide synthase distribution in the olfactory system of vertebrates.