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Titel
LAMPhimerus: a novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples
Autor(es)
Schlagwort
Parasitology
Microbiology, immunology, infectious diseases
Polymerase chain reaction
Diagnostic medicine
Fecha de publicación
2017
Verlag
Public Library of Science (New York)
Citación
Cevallos, W. Fernández-Soto, P. Calvopiña, M. Fontecha-Cuenca, C. Sugiyama, H. Sato, M. et al. (2017). LAMPhimerus: a novel LAMP assay for detecting Amphimerus sp. DNA in human stool samples. PLoS Negl Trop Dis 11(6): e0005672. https://doi.org/10.1371/journal.pntd.0005672
Resumen
[EN]Amphimeriasis, a fish-borne zoonotic disease caused by the liver fluke Amphimerus spp.,
is a highly prevalent parasitic infection affecting an indigenous Amerindian group, the
Chachi, living in rural and remote tropical areas along the RõÂo Cayapas and its tributaries
in the north-western coastal rainforest of Ecuador. Very little is known about the clinical
course and treatment of this disease, and the only method for diagnosing it is the parasitological
microscopic detection of eggs from Amphimerus spp. in patients' stool samples.
This method lacks sensitivity, and the morphology of the eggs may be confounded with
other liver and intestinal flukes. New diagnostic tools that can improve the sensitivity and
specificity for diagnosing Amphimerus spp. infection would be desirable. At present,
LAMP technology shows all the characteristics required of a real-time assay with simple
operation for potential use in the clinical diagnosis of infectious diseases, particularly in
the field conditions in developing countries for most neglected tropical diseases. In this
study, we developed and successfully evaluated a LAMP assay for detecting Amphimerus
sp. in human stool samples. After further validation, our LAMP assay (LAMPhimerus)
could be readily adapted for effective field diagnosis and disease surveillance in amphimeriasis-
endemic areas.
URI
ISSN
1935-2727
Versión del editor
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