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Titre
CRISPR-ERA for switching off (onco)genes
Autor(es)
Sujet
CRISPR
Fecha de publicación
2018
Éditeur
InTechOpen Book. Aditi Singh
Resumen
[EN]Genome editing nucleases like the popular CRISPR/Cas9 allow generate knock
-
out cell lines and nulls zygotes
by inducing site
-
specific DSB within a genome. In most cases, when a DNA template is not present, the DSB is
repaired by
non
-
homologous
end joining (NHEJ) resulting in small nucleotide insertions or deletions that can
be used to construct knockout alleles. However, for se
veral reasons, these mutations do not produce the
desired null result in all cases, generating a similar protein with functional activity. That undesirable effect
could limit the therapeutic efficiency of gene therapy strategies focused on abrogating oncog
ene expression by
CRISPR/Cas9 and should be taken in account. This chapter reviews the irruption of CRISPR technology for
gene silencing and its application in gene therapy.
URI
Aparece en las colecciones
- GMO. Monografías [1]