Compartir
Título
Nucleosomal signatures impose nucleosome positioning in coding and noncoding sequences in the genome
Otros títulos
Nucleosomal signatures and nucleosome positioning
Autor(es)
Assunto
Instituto de Biología Funcional y Genómica
Schizosaccharomyces and in Saccharomyces cerevisiae
Genómica
DNA
Nucleosomal organization
Fecha de publicación
2016
Citación
González, S., García, A., Vázquez, E., Serrano, R., Sánchez, M., Quintales, L., & Antequera, F. (2016). Nucleosomal signatures impose nucleosome positioning in coding and noncoding sequences in the genome. Genome research, 26(11), 1532-1543.
Resumen
[EN] In the yeast genome, a large proportion of nucleosomes occupy well-defined and stable positions. While the contribution of
chromatin remodelers and DNA binding proteins to maintain this organization is well established, the relevance of the DNA
sequence to nucleosome positioning in the genome remains controversial. Through quantitative analysis of nucleosome positioning,
we show that sequence changes distort the nucleosomal pattern at the level of individual nucleosomes in three
species of Schizosaccharomyces and in Saccharomyces cerevisiae. This effect is equally detected in transcribed and nontranscribed
regions, suggesting the existence of sequence elements that contribute to positioning. To identify such elements, we incorporated
information from nucleosomal signatures into artificial synthetic DNA molecules and found that they generated
regular nucleosomal arrays indistinguishable from those of endogenous sequences. Strikingly, this information is speciesspecific
and can be combined with coding information through the use of synonymous codons such that genes from
one species can be engineered to adopt the nucleosomal organization of another. These findings open the possibility of
designing coding and noncoding DNA molecules capable of directing their own nucleosomal organization.
URI
ISSN
1088-9051
DOI
10.1101/gr.207241.116
Versión del editor
Aparece en las colecciones