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dc.contributor.authorArévalo, Juan
dc.contributor.authorHernández-Jiménez, Enrique
dc.contributor.authorJiménez-González, Ada
dc.contributor.authorTorres-Valle, María
dc.contributor.authorIwasaki, Roman
dc.contributor.authorLópez-Bellido, Roger
dc.contributor.authorVicente-García, Cristina
dc.contributor.authorRodríguez, Raquel
dc.date.accessioned2020-01-31T09:13:43Z
dc.date.available2020-01-31T09:13:43Z
dc.date.issued2018
dc.identifier.citationArévalo, J. C., Hernández-Jiménez, E., Jiménez-González, A., Torres-Valle, M., Iwasaki, R. S., López-Bellido, R., ... & Rodríguez, R. E. (2018). Generation and Characterization of Antibodies against Opioid Receptors from Zebrafish. International journal of molecular sciences, 19(1), 14.es_ES
dc.identifier.urihttp://hdl.handle.net/10366/140748
dc.description.abstract[EN] The opioid system is well conserved among species and plays a critical role in pain and addiction systems. The use of zebrafish as an experimental model to study development and genetics is extraordinary and has been proven to be relevant for the study of different diseases. The main drawback to its use for the analysis of different pathologies is the lack of protein tools. Antibodies that work in other models are not suitable for zebrafish due to the low degree of homology that exists among the opioid receptor protein sequences in different species. Here we report the successful generation and characterization of antibodies against the mu, delta 1 and delta 2 opioid receptors in zebrafish. The antibodies obtained, which are specific for each receptor due to the use of the C-terminus as antigens, work for Western blotting and immunohistochemistry. In addition, the antibodies against mu and delta 1 opioid receptors, but not those against delta 2, are able to immunoprecipitate the corresponding receptor from zebrafish lysates. The development of opioid receptor antibodies is an asset to the further study of the endogenous opioid system in zebrafish.es_ES
dc.format.mimetypeapplicatio/pdf
dc.language.isoenges_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectOpioid receptorses_ES
dc.subjectAntibodieses_ES
dc.subjectZebrafishes_ES
dc.subjectImmunologyes_ES
dc.subject.meshAntibodies*
dc.subject.meshPsychoneuroimmunology*
dc.titleGeneration and Characterization of Antibodies against Opioid Receptors from Zebrafishes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publishversionhttp://dx.doi.org/10.3390/ijms19010014
dc.identifier.doi10.3390/ijms19010014
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.essn1422-0067
dc.journal.titleInternational Journal of Molecular Scienceses_ES
dc.volume.number19es_ES
dc.issue.number1es_ES
dc.page.initial1es_ES
dc.page.final12es_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES
dc.subject.decspsiconeuroinmunología*
dc.subject.decsanticuerpos*


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional