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Título
C. elegans Integrator complex: Identification and analysis of a role beyond snRNA processing
Autor(es)
Director(es)
Materia
Tesis y disertaciones académicas
Universidad de Salamanca (España)
Tesis Doctoral
Academic dissertations
Estudio
ADN
Clasificación UNESCO
2414 Microbiología
Fecha de publicación
2017
Resumen
[EN] In this work we described the C. elegans Integrator complex and the involvement of
its INTS-6 subunit in DNA damage response.
The Integrator complex, which is comprised of at least fourteen subunits in human
cells, is responsible for snRNA 3’-end processing. In addition, some of its subunits
are involved in other steps of the RNAP II transcription cycle or other biological
processes such as development or DNA repair.
Here, we demonstrated that the C. elegans Integrator complex is comprised of, at
least, eleven subunits (INTS-1, INTS-2, INTS-3, INTS-4, INTS-5, INTS-6, INTS-7,
INTS-8, INTS-9, INTS-11 and INTS-13). RNAi knockdown of any subunit leads to 3’-
end processing defects that result in the formation of chimeric RNAs composed of an
snRNA and an mRNA, which we have called “sn-mRNAs”’.
We also detected these chimeric “sn-mRNAs” upon gamma radiation. Finally,
involvement of INTS-6 in DNA repair via the HR pathway was demosnstrated and we
suggested a link between INTS-6 in DSB DNA repair and the formation of chimeric
“sn-mRNAs”.
URI
DOI
10.14201/gredos.151034
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