Compartir
Título
Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
Autor(es)
Materia
Cellulase CelC2
Rhizobium
Trifolium
Medicago
Hot phenotype
Ensifer meliloti
Clasificación UNESCO
2414 Microbiología
Fecha de publicación
2019
Editor
Springer Nature
Citación
Menéndez, E., Robledo, M., Jiménez-Zurdo, J. I., Velázquez, E., Rivas, R., Murray, J. D., & Mateos, P. F. (2019). Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection. Scientific Reports, 9(1), 13907. doi:10.1038/s41598-019-50337-3
Resumen
[EN]Primary infection of legumes by rhizobia involves the controlled localized enzymatic breakdown of cell
walls at root hair tips. Previous studies determined the role of rhizobial CelC2 cellulase in different steps
of the symbiotic interaction Rhizobium leguminosarum-Trifolium repens. Recent findings also showed
that CelC2 influences early signalling events in the Ensifer meliloti-Medicago truncatula interaction.
Here, we have monitored the root hair phenotypes of two legume plants, T. repens and M. sativa,
upon inoculation with strains of their cognate and non-cognate rhizobial species, R. leguminosarum
bv trifolii and E. meliloti, (over)expressing the CelC2 coding gene, celC. Regardless of the host, CelC2
specifically elicited ‘hole-on-the-tip’ events (Hot phenotype) in the root hair apex, consistent with the
role of this endoglucanase in eroding the noncrystalline cellulose found in polarly growing cell walls.
Overproduction of CelC2 also increased root hair tip redirections (RaT phenotype) events in both
cognate and non-cognate hosts. Interestingly, heterologous celC expression also induced non-canonical
alterations in ROS (Reactive Oxygen Species) homeostasis at root hair tips of Trifolium and Medicago.
These results suggest the concurrence of shared unspecific and host-related plant responses to CelC2
during early steps of symbiotic rhizobial infection. Our data thus identify CelC2 cellulase as an important
determinant of events underlying early infection of the legume host by rhizobia.
Descripción
Article number: 13907 (2019)
URI
DOI
10.1038/s41598-019-50337-3
Versión del editor
Colecciones