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Título
Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test
Autor(es)
Materia
Molecular Diagnostic Techniques
Schistosoma mansoni
loop-mediated isothermal amplification
Point-of-Care Systems
Clasificación UNESCO
24 Ciencias de la vida
3205.05 Enfermedades Infecciosas
Fecha de publicación
2019-10-14
Editor
Nature Publishing Group
Citación
García-Bernalt Diego, J., Fernández-Soto, P., Crego-Vicente, B., Alonso-Castrillejo, S., Febrer-Sendra, B., Gómez-Sánchez, A., ... & Muro, A. (2019). Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test. Scientific Reports, 9(1), 14744.
Resumen
[ENG]Schistosomiasis is one of the most prevalent Neglected Tropical Disease, affecting approximately 250 million people worldwide. Schistosoma mansoni is the most important species causing human intestinal schistosomiasis. Despite significant efforts in recent decades, the global disease burden of schistosomiasis remains extremely high. This could partly be attributed to the absence of accurate diagnostic tools, primarily in endemic areas. Loop-mediated isothermal amplification (LAMP) is increasingly used in molecular diagnostics as a field-friendly alternative to many other complex molecular methods and it has been proposed as an ideal candidate for revolutionizing point-of-care molecular diagnostics. In a previous work, a LAMP-based method to detect S. mansoni DNA (SmMIT-LAMP) was developed by our research group for early diagnosis of active schistosomiasis in an experimental infection murine model. The SmMIT-LAMP has been further successfully evaluated in both human stool and snail samples and, recently, in human urine samples. In this study, we developed an important improvement for SmMIT-LAMP molecular assay, transforming it into a cold maintenance dry format suitable for potentially manufacturing as kit for ready-to-use for schistosomiasis diagnosis. This procedure could be applied to create dry LAMP kits for a laboratory setting and for diagnostic applications for other neglected tropical diseases.
URI
DOI
10.1038/s41598-019-51342-2
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