Título
Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization-time of flight mass spectrometry
Autor(es)
Palabras clave
Infección tracto urinario
Bacterias
Fecha de publicación
2014-01
Editor
American Society for Microbiology
Citación
Sánchez-Juanes, F., Siller Ruiz, M., Moreno Obregon, F., Criado Gonzalez, M., Hernandez Egido, S., de Frutos Serna, M., ... & Muñoz-Bellido, J. L. (2014). Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization–time of flight mass spectrometry. Journal of clinical microbiology, 52(1), 335-338. DOI: https://doi.org/10.1128/jcm.01881-13
Resumen
[EN]We pretreated with SDS 71 urine samples with bacterial counts of >10(5) CFU/ml and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identification scores of <2, in order to minimize failure rates. Identification improved in 46.5% of samples, remained unchanged in 49.3%, and worsened in 4.2%. The improvement was more evident for Gram-negative (54.3%) than for Gram-positive (32%) bacteria.
URI
ISSN
0095-1137
DOI
Versión del editor
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