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Título
CRB2 completes a fully expressed Crumbs complex in the Retinal Pigment Epithelium
Autor(es)
Materia
Adherens junctions
Apicobasal polarity
Cellular neuroscience
Tight junctions
Clasificación UNESCO
2490 Neurociencias
Fecha de publicación
2015
Citación
Paniagua, A.E., Herránz Martín, S., Jimeno, D., Jimeno, Á.M., López Benito, S., Arévalo, J.C., Velasco Arranz, A., Aijón Noguera, J. & Lillo Delgado, C. (2015). CRB2 completes a fully expressed Crumbs complex in the Retinal Pigment Epithelium. Scientific Reports 5, 14504. https://doi.org/10.1038/srep14504
Resumen
The CRB proteins CRB1, CRB2 and CRB3 are members of the cell polarity complex Crumbs in mammals that together with Scribble and Par complexes stablish the polarity of a variety of cell types. Although many members of the Crumbs complex proteins are expressed in the retinal pigment epithelium (RPE) and even though the mRNA of CRB2 has been detected in ARPE-19 cells and in the RPE/Choroid, to date no CRB protein has yet been found in this tissue. To investigate this possibility, we generated an antibody that specifically recognize the mouse CRB2 protein and we demonstrate the expression of CRB2 in mouse RPE. Confocal analysis shows that CRB2 is restricted to the apicolateral membrane of RPE cells and more precisely, in the tight junctions. Our study identified CRB2 as the member of the CRB protein family that is present together with the rest of the components of the Crumbs complex in the RPE apico-lateral cell membrane. Considering that the functions of CRB proteins are decisive in the establishment and maintenance of cell-cell junctions in several epithelial-derived cell types, we believe that these findings are a relevant starting point for unraveling the functions that CRB2 might perform in the RPE.
URI
DOI
10.1038/srep14504
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