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dc.contributor.author | van Dongen, J J M | |
dc.contributor.author | Langerak, A W | |
dc.contributor.author | Brüggemann, M | |
dc.contributor.author | Evans, P A S | |
dc.contributor.author | Hummel, M | |
dc.contributor.author | Lavender, F L | |
dc.contributor.author | Delabesse, E | |
dc.contributor.author | Davi, F | |
dc.contributor.author | Schuuring, E | |
dc.contributor.author | García Sanz, Ramón | |
dc.contributor.author | van Krieken, J H J M | |
dc.contributor.author | Droese, J | |
dc.contributor.author | González, D | |
dc.contributor.author | Bastard, C | |
dc.contributor.author | White, H E | |
dc.contributor.author | Spaargaren, M | |
dc.contributor.author | González Díaz, Marcos | |
dc.contributor.author | Parreira, A | |
dc.contributor.author | Smith, J L | |
dc.contributor.author | Morgan, G J | |
dc.contributor.author | Kneba, M | |
dc.contributor.author | Macintyre, E A | |
dc.date.accessioned | 2024-02-05T10:21:49Z | |
dc.date.available | 2024-02-05T10:21:49Z | |
dc.date.issued | 2003-12 | |
dc.identifier.citation | Van Dongen, J. J. M., Langerak, A. W., Brüggemann, M., Evans, P. A. S., Hummel, M., Lavender, F. L., ... & Macintyre, E. A. (2003). Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936. Leukemia, 17(12), 2257-2317. doi:10.1038/sj.leu.2403202 | es_ES |
dc.identifier.issn | 0887-6924 | |
dc.identifier.uri | http://hdl.handle.net/10366/155309 | |
dc.description | Es el artículo principal en el desarrollo, estandarización y aplicación de la biología molecular para el diagnóstico de clonalidad en síndromes linfoproliferativos B y T. Hoy en día, práctica-mente todos los diagnósticos de linfomas y Síndromes relacionados que se diagnostican en Europa y en USA utilizan esta metodología como ayuda para en el diagnóstico definitivo. | es_ES |
dc.description.abstract | [EN]In a European BIOMED-2 collaborative study, multiplex PCR assays have successfully been developed and standardized for the detection of clonally rearranged immunoglobulin (Ig) and T-cell receptor (TCR) genes and the chromosome aberrations t(11;14) and t(14;18). This has resulted in 107 different primers in only 18 multiplex PCR tubes: three VH-JH, two DH-JH, two Ig kappa (IGK), one Ig lambda (IGL), three TCR beta (TCRB), two TCR gamma (TCRG), one TCR delta (TCRD), three BCL1-Ig heavy chain (IGH), and one BCL2-IGH. The PCR products of Ig/TCR genes can be analyzed for clonality assessment by heteroduplex analysis or GeneScanning. The detection rate of clonal rearrangements using the BIOMED-2 primer sets is unprecedentedly high. This is mainly based on the complementarity of the various BIOMED-2 tubes. In particular, combined application of IGH (VH-JH and DH-JH) and IGK tubes can detect virtually all clonal B-cell proliferations, even in B-cell malignancies with high levels of somatic mutations. The contribution of IGL gene rearrangements seems limited. Combined usage of the TCRB and TCRG tubes detects virtually all clonal T-cell populations, whereas the TCRD tube has added value in case of TCRgammadelta(+) T-cell proliferations. The BIOMED-2 multiplex tubes can now be used for diagnostic clonality studies as well as for the identification of PCR targets suitable for the detection of minimal residual disease. | es_ES |
dc.description.sponsorship | Hospital Universitario de Salamanca Universidad de Salamamnca | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Nature | es_ES |
dc.subject | Lymphoma | es_ES |
dc.subject | Rearrangement | es_ES |
dc.subject | Immunoglobulin | es_ES |
dc.subject | T-cell receptor | es_ES |
dc.subject | Monoclonality | es_ES |
dc.subject.mesh | Chromosome Aberrations | * |
dc.subject.mesh | Lymphoproliferative Disorders | * |
dc.subject.mesh | European Union | * |
dc.subject.mesh | Clone Cells | * |
dc.subject.mesh | DNA Primers | * |
dc.subject.mesh | Gene Rearrangement | * |
dc.subject.mesh | Humans | * |
dc.subject.mesh | Polymerase Chain Reaction | * |
dc.subject.mesh | Reference Standards | * |
dc.subject.mesh | Reproducibility of Results | * |
dc.subject.mesh | Immunoglobulins | * |
dc.title | Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 Concerted Action BMH4-CT98-3936 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publishversion | https://www.nature.com/articles/2403202 | es_ES |
dc.subject.unesco | 3205.04 Hematología | es_ES |
dc.identifier.doi | 10.1038/sj.leu.2403202 | |
dc.relation.projectID | BIOMED-2 Concerted Action BMH4-CT98-3936 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.identifier.pmid | 14671650 | |
dc.identifier.essn | 1476-5551 | |
dc.journal.title | Leukemia | es_ES |
dc.volume.number | 17 | es_ES |
dc.issue.number | 12 | es_ES |
dc.page.initial | 2257 | es_ES |
dc.page.final | 2317 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.decs | reacción en cadena de la polimerasa | * |
dc.subject.decs | humanos | * |
dc.subject.decs | trastornos linfoproliferativos | * |
dc.subject.decs | reproducibilidad de resultados | * |
dc.subject.decs | células clonales | * |
dc.subject.decs | aberraciones cromosómicas | * |
dc.subject.decs | inmunoglobulinas | * |
dc.subject.decs | cebadores de ADN | * |
dc.subject.decs | estándares de referencia | * |
dc.subject.decs | reordenamiento génico | * |
dc.subject.decs | Unión Europea | * |
dc.description.project | Hospital Universitario de Salamanca | es_ES |