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Título
Pectic galactan affects cell wall architecture during secondary cell wall deposition
Autor(es)
Materia
β-(1,4)-Galactan
β-Galactosidase
Arabidopsis
Cell wall
Hemicellulose
Fecha de publicación
2020
Resumen
Despite recent advances regarding the role of pectic β-(1,4)-galactan neutral side chains in primary cell wall remodelling
during growth and cell elongation, little is known about the specific function of this polymer in other developmental
processes. We have used transgenic Arabidopsis plants overproducing chickpea βI-Gal β-galactosidase under the 35S CaMV
promoter (35S::βI-Gal) with reduced galactan levels in the basal non-elongating floral stem internodes to gain insight into the
role of β-(1,4)-galactan in cell wall architecture during the cessation of elongation and the beginning of secondary growth.
The loss of galactan mediated by βI-Gal in 35S::βI-Gal plants is accompanied by a reduction in the levels of KOH-extracted
xyloglucan and an increase in the levels of xyloglucan released by a cellulose-specific endoglucanase. These variations in
cellulose–xyloglucan interactions cause an altered xylan and mannan deposition in the cell wall that in turn results in a deficient
lignin deposition. Considering these results, we can state that β-(1,4)-galactan plays a key structural role in the correct
organization of the different domains of the cell wall during the cessation of growth and the early events of secondary cell
wall development. These findings reinforce the notion that there is a mutual dependence between the different polysaccharides
and lignin polymers to form an organized and functional cell wall.
URI
ISSN
0032-0935
DOI
10.1007/s00425-020-03394-2
Versión del editor
Colecciones
- GIPACYCV. Artículos [58]
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