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Título
The Location of the Chickpea Cell Wall ßV-Galactosidase Suggests Involvement in the Transition between Cell Proliferation and Cell Elongation
Autor(es)
Materia
Cell wall
Chickpea
ß-galactosidase
Elongation
Meristem
Fecha de publicación
2009
Resumen
We report the generation of antibodies against
a ß-galactosidase (ßV-Gal) from Cicer arietinum and the
subsequent immunolocalization of the protein in different
parts and developmental stages of the plant. ßV-Gal is a
cell wall protein encoded by the CanBGal-5 gene, which
belongs to a family of at least four ß-galactosidase genes in
chickpea. We have previously reported that CanBGal-5
transcripts are located in organs with high elongation and
cell division rates, such as meristematic hooks, very young
epicotyls, and apical internodes. ßV-Gal protein is the only
studied chickpea ß-galactosidase widely present in meristematic
hooks, mainly in the meristematic apical zone.
These results agree with the previously reported transcription
pattern of CanBGal-5 and may reflect its
involvement in cell wall modifications during the final
stages of cell proliferation, leading to the establishment of
an expanding cell wall. The location of ßV-Gal in the cell
wall of procambium cells and in pericycle cells of the
developing lateral roots also supports the involvement of
ßV-Gal in this process. During seedling and plant growth,
the highest levels of bV-Gal protein were detected in the
youngest actively growing epicotyls and in the apical
growing internodes. Thus, protein levels pointed to a
relationship between bV-Gal and the events occurring in
the cell wall during the early stages of development. Immunolocalization studies in different zones of epicotyls
and radicles suggest a role for ßV-Gal in cell elongation.
URI
ISSN
0721-7595
DOI
10.1007/s00344-008-9067-2
Colecciones
- GIPACYCV. Artículos [58]
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