| dc.contributor.author | Martín Sánchez, José Ignacio | |
| dc.contributor.author | Dopico, Berta | |
| dc.contributor.author | Muñoz, Francisco J. | |
| dc.contributor.author | Esteban Gallego, María Rocío | |
| dc.contributor.author | Oomen, Ronald J. F. J. | |
| dc.contributor.author | Driouich, Azeddine | |
| dc.contributor.author | Vincken, Jean-Paul | |
| dc.contributor.author | Visser, Richard | |
| dc.contributor.author | Labrador, Emilia | |
| dc.date.accessioned | 2024-04-03T06:38:33Z | |
| dc.date.available | 2024-04-03T06:38:33Z | |
| dc.date.issued | 2005 | |
| dc.identifier.issn | 0032-0781 | |
| dc.identifier.uri | http://hdl.handle.net/10366/156954 | |
| dc.description.abstract | We report the generation of Solanum tuberosum transformants
expressing Cicer arietinum βIII-Gal. βIII-Gal is a
β-galactosidase able to degrade cell wall pectins during cell
wall loosening that occurs prior to cell elongation. cDNA
corresponding to the gene encoding this protein was identified
among several chickpea β-galactosidase cDNAs, and
named CanBGal-3. CanBGal-3 cDNA was expressed in
potato under the control of the granule-bound starch
synthase promoter. Three βIII-Gal transformants with varying
levels of expression were chosen for further analysis.
The transgenic plants displayed no significant altered phenotype
compared to the wild type. However, β-galactanase
and β-galactosidase activities were increased in the transgenic
tuber cell walls and this affected the potato tuber
pectins. A reduction in the galactosyl content of up to 50%
compared to the wild type was observed in the most
extreme transformant, indicating a reduction of 1,4-β-
galactan side-chains, as revealed by analysis with LM5 specific
antibodies. Our results confirm the notion that the
pectin-degrading activity of chickpea βIII-Gal reported in
vitro also occurs in vivo and in other plants, and confirm
the involvement of βIII-Gal in the cell wall autolysis process.
An increase in the homogalacturonan content of transgenic
tuber cell walls was also observed by Fourier
transform infrared spectroscopy (FTIR) analysis. | es_ES |
| dc.description.sponsorship | This work was supported by a grant from the
EC (CT97 2224) and a grant from the Dirección General de Investigación,
Spain (BOS2002–01900). | es_ES |
| dc.language.iso | eng | es_ES |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Cell wall | es_ES |
| dc.subject | Chickpea | es_ES |
| dc.subject | β-galactosidase | es_ES |
| dc.subject | Pectin | es_ES |
| dc.subject | Potato | es_ES |
| dc.subject | Transgenic plant | es_ES |
| dc.title | In vivo Expression of a Cicer arietinum β-galactosidase in Potato Tubers Leads to a Reduction of the Galactan Side-chains in Cell Wall Pectin | es_ES |
| dc.type | info:eu-repo/semantics/article | es_ES |
| dc.identifier.doi | 10.1093/pcp/pci177 | |
| dc.relation.projectID | CT97 222 | es_ES |
| dc.relation.projectID | BOS2002–01900 | es_ES |
| dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
| dc.identifier.essn | 1471-9053 | |
| dc.journal.title | Plant and Cell Physiology | es_ES |
| dc.volume.number | 46 | es_ES |
| dc.issue.number | 10 | es_ES |
| dc.page.initial | 1613 | es_ES |
| dc.page.final | 1622 | es_ES |
| dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |