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dc.contributor.author | Dopico, Berta | |
dc.contributor.author | Nicolás Rodrigo, Gregorio | |
dc.contributor.author | Labrador, Emilia | |
dc.date.accessioned | 2024-04-16T08:09:36Z | |
dc.date.available | 2024-04-16T08:09:36Z | |
dc.date.issued | 1991 | |
dc.identifier.issn | 0176-1617 | |
dc.identifier.uri | http://hdl.handle.net/10366/157389 | |
dc.description.abstract | In the cell walls of epicotyls of Cicer arietinum we found beta-glucosidase and a-galactosidase activities, the former being predominant. We purified and characterized these enzymatic activities. The beta-glucosidase activity is found associated with a protein fraction that also shows beta-galactosidase and beta-xylosidase activity. lt is formed by two subunits of approximately 60-65 kDa, with elevated activity between pH 6 and 8 and an optima! temperature of 70 ºC, inhibited by Hg2+ . The values of Km and Vmax vary for each of its principal activities, being 2.2 mM and 378.6 nkat (mg protein)- 1, respectively, for beta-glucosidase activity. The fraction with the a-galactosidase activity is also formed by subunits of molecular weight of 35-45 kDa. lt shows an optimum pH of 5 and 40 ºC, its Km and V max being 0.27 mM and 32.8 nkat (mg protein)- 1. lt is inhibited by Hg2+ and galactose. Throughout growth of the epicotyls, beta-glucosidase activity increased and a-galactosidase showed an activity profile with a maximum to the 5th day. These enzymes were not able to hydrolyze the non-cellulosic fractions of the cell wall. lts physiological role remains unknown. | es_ES |
dc.description.sponsorship | This work was supported by a grant from Direcci6n General de lnvestigaci6n Científica y Técnica, Spain nº PB87-0024 | es_ES |
dc.language.iso | eng | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Cicer arietinum L. | es_ES |
dc.subject | Cell wall alpha-galactosidase | es_ES |
dc.subject | Cell wall beta-glucosidase | es_ES |
dc.subject | Growth | es_ES |
dc.subject | Hydrolysis | es_ES |
dc.title | Characterization, Hydrolytic Activity and Variations throughout Growth of a Cell Wall β-Glucosidase and a-Galactosidase from Cicer arietinum epicotyls | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1016/S0176-1617(11)80320-2 | |
dc.relation.projectID | PB87-0024 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.journal.title | Journal of Plant Physiology | es_ES |
dc.volume.number | 137 | es_ES |
dc.issue.number | 4 | es_ES |
dc.page.initial | 477 | es_ES |
dc.page.final | 482 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
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