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dc.contributor.authorDopico, Berta 
dc.contributor.authorNicolás Rodrigo, Gregorio
dc.contributor.authorLabrador, Emilia 
dc.date.accessioned2024-04-16T08:10:22Z
dc.date.available2024-04-16T08:10:22Z
dc.date.issued1990
dc.identifier.issn0031-9317
dc.identifier.urihttp://hdl.handle.net/10366/157391
dc.description.abstractbeta-Galactosidase (EC 3.2.1.23) has been established as the main enzyme involved in the autolytic process. The enzyme extracted from cell walls of epicotyls of Cicer arietinum L. cv. Castellana with 3 M LiCI is a 45 kDa protein composed of a single subunit, having an optimum pH of 4; an optimum temperature of 45ºC and Km and V of 1.72 mM and 18.5 nkat (mg proteint' respectively, as evaluated against p-nitrophenyl-beta-D-galactopyranoside. The enzyme is inhibited by Hg2+, o-galac­tono-1,4-lactone and galactose, substances that also inhibit the autolytic process. Ca2+ and EDTA, which do not affect the activity of the beta-galactosidase, do however modify the hydrolysis of the cell wall mediated by the enzyme, and they also inhibit the autolytic process. Ca2+ decreased both processes, whereas EDTA increased them; and when both substances were added together, their individual effects were neutralized. The effects of both agents is probably due to modifications in the cell wall that prevent access of the enzyme to its substrate.es_ES
dc.description.sponsorshipThis work was supported by a grant from the Dirección General de Investigación Científica y Técnica, Spain, nº PB87-0024.es_ES
dc.language.isoenges_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCell walles_ES
dc.subjectChick-peaes_ES
dc.subjectCicer arietinumes_ES
dc.subjectBeta-galactosidasees_ES
dc.titleCharacterization of a cell wall β‐galactosidase of Cicer arietinum epicotyls involved in cell wall autolysises_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1111/j.1399-3054.1990.tb05689.x
dc.relation.projectIDPB87-0024es_ES
dc.rights.accessRightsinfo:eu-repo/semantics/embargoedAccesses_ES
dc.identifier.essn1399-3054
dc.journal.titlePhysiologia Plantarumes_ES
dc.volume.number80es_ES
dc.issue.number4es_ES
dc.page.initial629es_ES
dc.page.final635es_ES
dc.type.hasVersioninfo:eu-repo/semantics/publishedVersiones_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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