Compartir
Título
Characterization of GFAP expression and cell proliferation in the rat median eminence following hypophysectomy
Autor(es)
Materia
Median eminence
GFAP-cells
Cellular proliferation
Hypophysectomy
lmmunohistochemistry
Fecha de publicación
2001
Resumen
[EN]To analyze whether the reorganization of the
rat median eminence after hypophysectomy might be
related to changes in glial fibrillary acidic protein
(GFAP)- and cellular proliferation, the distribution of
cells immunoreactive for GFAP and the proliferation rate
of such cells were analyzed at 20, 40 and 60 days posthypophysectomy.
For this study, four rostro-caudal
regions of the median eminence were differentiated: the
retrochiasmatic, preinfundibular, infundibular and
postinfundibular regions. In each of these regions, three
layers were studied: the ependymal, the internal and the
external. At 20 and 40 days after hypophysectomy,
significant increases in cellular proliferation affecting all
three layers studied in the preinfundibular and
infundibular regions were found. At the same time
points, increases in GFAP expression were also
observed. However, after 60 days, GFAP and
proliferative cellular nuclear antigen (PCNA) expression
decreased. Although variations of PCNA and GFAP
levels were evident, no colocalisation of PCNA and
GFAP was found in the cells of the median eminence in
untreated or hypophysectomized rats when sections were
analyzed by double immunohistochemical staining. Our
results suggest that reorganization of median eminence
involves alterations (or modulation) of GFAPimmunoreactive
cells together with a proliferation of
cells that are not GFAP-immunoreactive. This study also
demonstrates that this reorganization is completed within
the first two months after hypophysectomy.
URI
ISSN
0213-3911
Colecciones