Título
Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma
Autor(es)
Palabras clave
Constitutive gene
Expression analysis
Reference gene
Normalization
qPCR
gene
Validation reference gene
Clasificación UNESCO
Fecha de publicación
2016
Editor
Humana Press, New York, NY
Citación
San Segundo-Val, I., & Sanz-Lozano, C. S. (2016). Validation of reference genes in mRNA expression analysis applied to the study of asthma. Molecular Genetics of Asthma, 57-69.
Resumen
[EN] The quantitative Polymerase Chain Reaction is the most used technique for the study of gene expression. To correct putative experimental errors of this technique is necessary normalizing the expression results of the gene of interest with the obtained for reference genes. Here, we describe an example of the process to select reference genes. In this particular case, we select reference genes for expression studies in the peripheral blood mononuclear cells of asthmatic patients.
Descripción
URI
ISBN
978-1-4939-3650-2
ISSN
1064-3745
DOI
10.1007/978-1-4939-3652-6_5
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