Título
Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma
Autor(es)
Palabras clave
Binding
Electrophoretic mobility shift assay
EMSA
Gel retardation
Protein–DNA complex
Clasificación UNESCO
Fecha de publicación
2016
Editor
Humana Press, New York, NY
Citación
García-Solaesa, V., & Sanz-Lozano, C. S. (2016). Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma. Molecular Genetics of Asthma, 91-105.
Resumen
[EN] Electrophoretic mobility shift assays (EMSA) are used to characterize interactions between nucleic acids and proteins in native conditions. This is based on the fact that the electrophoretic mobility of a nucleic acid becomes slower when it forms complexes with proteins. There are many different variants and applications of this methodology. In this chapter we describe a detailed EMSA protocol applied to the study of asthma.
Descripción
URI
ISBN
978-1-4939-3650-2
ISSN
1064-3745
DOI
10.1007/978-1-4939-3652-6_7
Versión del editor
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