2026-09-15T21:25:06Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1287952025-06-05T12:40:28Zcom_10366_4577com_10366_4576com_10366_3823com_10366_4756com_10366_4746col_10366_4585col_10366_68517

Rivas Sanz, Javier de las Sánchez-Guijo Martín, Fermín Cañizo Fernández-Roldán, María Consuelo del Rosón Burgo, Beatriz 2016-05-25T07:17:09Z 2016-05-25T07:17:09Z 2015 http://hdl.handle.net/10366/128795 10.14201/gredos.128795 [EN] The therapeutically applied population of Mesenchymal Stromal/Stem Cells, defined under the minimal criteria of the International Society of Cellular Therapy (ISCT), have still a defectively characterized phenotype difficult to distinguish from similar cell populations. This Doctoral Thesis has been formulated as a characterizing data-driven approach. Thus, the main scope is to improve the characterization of the phenotype of human mesenchymal stem/stromal cells. We have approached such scope through a deep comparative transcriptomic study applying high-throughput genome-wide analytic techniques: producing and integrating RNA sequencing and microarray data assays, with a meta-analysis of a large collection of public expression data. The research falls within the fields of cellular, molecular and systems biology, which entails two complementary approaches: the experimental, and the computational ones. First, we isolated, cultured and validated (following the ISCT criteria of immunophenotype and in-vitro tri-lineage differentiation) human MSCs from three tissue origins: placenta, bone marrow, and adipose tissue. Applying bioinformatics techniques, we then defined a gene expression signature common to all human tissue-MSCs, as well as the specific expression profiles associated to each tissue-MSC type. Based on theseachievements, we could come up with novel insights about the MSC phenotype that will lead to the drafting of new hypotheses. Also, we yielded a broad transcriptomic signature resource ready to serve as reference on upcoming identification studies. 185 p. application/pdf Inglés eng Attribution-NonCommercial-NoDerivs 3.0 Unported https://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Tesis y disertaciones académicas Universidad de Salamanca (España) Academic dissertations Biología humana Biología molecular Biología celular Bioestadística 2302.21 Biología Molecular 2407 Biología Celular 2410 Biología Humana 2404.01 Bioestadística Transcriptomic characterization of human Mesenchymal Stromal/Stem Cells info:eu-repo/semantics/doctoralThesis info:eu-repo/semantics/doctoralThesis