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oai:gredos.usal.es:10366/1287952025-06-05T12:40:28Zcom_10366_4577com_10366_4576com_10366_3823com_10366_4756com_10366_4746col_10366_4585col_10366_68517

Transcriptomic characterization of human Mesenchymal Stromal/Stem Cells Rosón Burgo, Beatriz Rivas Sanz, Javier de las Sánchez-Guijo Martín, Fermín Cañizo Fernández-Roldán, María Consuelo del [EN] The therapeutically applied population of Mesenchymal Stromal/Stem Cells, defined under the minimal criteria of the International Society of Cellular Therapy (ISCT), have still a defectively characterized phenotype difficult to distinguish from similar cell populations. This Doctoral Thesis has been formulated as a characterizing data-driven approach. Thus, the main scope is to improve the characterization of the phenotype of human mesenchymal stem/stromal cells. We have approached such scope through a deep comparative transcriptomic study applying high-throughput genome-wide analytic techniques: producing and integrating RNA sequencing and microarray data assays, with a meta-analysis of a large collection of public expression data. The research falls within the fields of cellular, molecular and systems biology, which entails two complementary approaches: the experimental, and the computational ones. First, we isolated, cultured and validated (following the ISCT criteria of immunophenotype and in-vitro tri-lineage differentiation) human MSCs from three tissue origins: placenta, bone marrow, and adipose tissue. Applying bioinformatics techniques, we then defined a gene expression signature common to all human tissue-MSCs, as well as the specific expression profiles associated to each tissue-MSC type. Based on theseachievements, we could come up with novel insights about the MSC phenotype that will lead to the drafting of new hypotheses. Also, we yielded a broad transcriptomic signature resource ready to serve as reference on upcoming identification studies. 2015 info:eu-repo/semantics/doctoralThesis info:eu-repo/semantics/doctoralThesis eng http://hdl.handle.net/10366/128795 https://gredos.usal.es/bitstream/10366/128795/11/CIC_Ros%c3%b3nBurgoB_Transcriptomiccharacterizationhuman.pdf.txt 573c7be2be84984780b6c5e581b6d43b https://gredos.usal.es/bitstream/10366/128795/13/CIC_Ros%c3%b3nBurgoB_TranscriptomiccharacterizationhumanResumen.pdf.txt f702e9f9086837eded78e81f8505dcca https://gredos.usal.es/bitstream/10366/128795/1/CIC_Ros%c3%b3nBurgoB_Transcriptomiccharacterizationhuman.pdf afdbcec9827e6e5f2ff60da9b4ca884c https://gredos.usal.es/bitstream/10366/128795/3/CIC_Ros%c3%b3nBurgoB_TranscriptomiccharacterizationhumanResumen.pdf 4a53db5fe7a4d9a09d4d13097cac1cef https://gredos.usal.es/bitstream/10366/128795/4/CIC_Ros%c3%b3nBurgoB_TranscriptomiccharacterizationhumanSupplemetaryMaterial.zip c8aadeb6bb9665e22c150f8598e21f40 https://gredos.usal.es/bitstream/10366/128795/2/license.txt 0d92de0f68b12270b041dd6003cea975 Attribution-NonCommercial-NoDerivs 3.0 Unported Tesis y disertaciones académicas Universidad de Salamanca (España) Academic dissertations Biología humana Biología molecular Biología celular Bioestadística 10.14201/gredos.128795