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oai:gredos.usal.es:10366/1407192025-04-30T19:33:01Zcom_10366_4001com_10366_3947com_10366_3946com_10366_3823col_10366_4002

Ferraz, Miriéle Cristina de Oliveira, Jhones Luiz de Oliveira Junior, Joel Reis Cogo, José Carlos dos Santos, Márcio Galdino Franco, Luiz Madaleno Puebla, Pilar Ferraz, Helena Onishi Ferraz, Humberto Gomes da Rocha, Marisa Maria Teixeira Hyslop, Stephen San Feliciano, Arturo Oshima-Franco, Yoko 2020-01-29T12:00:49Z 2020-01-29T12:00:49Z 2015 Ferraz, MC, de Oliveira, JL, de Oliveira Junior, JR, Cogo, JC, Dos Santos, MG, Franco, LM, Puebla, P, Ferraz, HO, Ferraz, HG, da Rocha, MM. (2015). The triterpenoid betulin protects against the neuromuscular effects of Bothrops jararacussu snake venom in vivo. Evidence-Based Complement and Alternative Medicine. Volume 2015, ID: 939523. http://dx.doi.org/10.1155/2015/939523 1741-427X http://hdl.handle.net/10366/140719 10.1155/2015/939523 1741-4288 [EN] We confirmed the ability of the triterpenoid betulin to protect against neurotoxicity caused by Bothrops jararacussu snake venom in vitro in mouse isolated phrenic nerve-diaphragm (PND) preparations and examined its capability of in vivo protection using the rat external popliteal/sciatic nerve-tibialis anterior (EPSTA) preparation. Venom caused complete, irreversible blockade in PND (40 𝜇g/mL), but only partial blockade (∼30%) in EPSTA (3.6mg/kg, i.m.) after 120 min. In PND, preincubation of venom with commercial bothropic antivenom (CBA) attenuated the venom-induced blockade, and, in EPSTA, CBA given i.v. 15 min after venom also attenuated the blockade (by ∼70% in both preparations). Preincubation of venom with betulin (200 𝜇g/mL) markedly attenuated the venom-induced blockade in PND; similarly, a single dose of betulin (20mg, i.p., 15 min after venom) virtually abolished the venom-induced decrease in contractility. Plasma creatine kinase activity was significantly elevated 120 min after venom injection in the EPSTA but was attenuated by CBA and betulin. These results indicate that betulin given i.p. has a similar efficacy as CBA given i.v. in attenuating the neuromuscular effects of B. jararacussu venom in vivo and could be a useful complementary measure to antivenom therapy for treating snakebite. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Triterpenoid Betulin Protects Bothrops jararacussu Neuromuscular Effects Snakebite The Triterpenoid Betulin Protects against the Neuromuscular Effects ofBothrops jararacussuSnake VenomIn Vivo info:eu-repo/semantics/article