2026-09-16T00:55:05Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1454852022-02-07T15:42:17Zcom_10366_4182com_10366_4055com_10366_3946com_10366_3823com_10366_137251com_10366_4512col_10366_4183col_10366_137252

García Estévez, Ignacio Escribano Bailón, María Teresa Alcalde Eon, Cristina 2021-03-03T10:26:10Z 2021-03-03T10:26:10Z 2017 García Estévez, I. [et al.] (2017). Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes. Journal of Agricultural and Food Chemistry, 65(31), pp. 6359-6368. doi: 10.1021/acs.jafc.6b05278 0021-8561 http://hdl.handle.net/10366/145485 10.1021/acs.jafc.6b05278 1520-5118 [EN] The determination of the detailed flavanol composition in food matrices is not a simple task because of the structural similarities of monomers and, consequently, oligomers and polymers. The aim of this study was the development and validation of an HPLC-MS/MS−multiple reaction monitoring (MRM) method that would allow the accurate and precise quantification of catechins, gallocatechins, and oligomeric proanthocyanidins. The high correlation coefficients of the calibration curves (>0.993), the recoveries not statistically different from 100%, the good intra- and interday precisions (<5%), and the LOD and LOQ values, low enough to quantify flavanols in grapes, are good results from the method validation procedure. Its usefulness has also been tested by determining the detailed composition of Vitis vinifera L. cv. Rufete grapes. Seventy-two (38 nongalloylated and 34 galloylated) and 53 (24 procyanidins and 29 prodelphinidins) flavanols have been identified and quantified in grape seed and grape skin, respectively. The use of HCA and PCA on the detailed flavanol composition has allowed differentiation among Rufete clones. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Quantitative mass spectrometry HPLC-DAD-MS-multiple reaction monitoring Flavanol Grape seed Grape skin Vitis vinifera L. cv. Rufete Clonal classification Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes info:eu-repo/semantics/article