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oai:gredos.usal.es:10366/1466752025-04-30T19:45:23Zcom_10366_4074com_10366_4055com_10366_3946com_10366_3823com_10366_133043com_10366_4576com_10366_4577col_10366_4075col_10366_135272col_10366_4578

00925njm 22002777a 4500 dc González-Tablas Pimenta, María author Crespo, Inês author Vital, Ana Luísa author Otero Rodríguez, Álvaro author Nieto Librero, Ana Belén author Sousa, Pablo author Patino Alonso, María Carmen author Corchete Sánchez, Luis Antonio author Tão, Hermínio author Rebelo, Olinda author Barbosa, Marcos author Almeida, Maria Rosário author Guedes, Ana Filipa author Lopes, María Celeste author French, Pim J. author Orfao de Matos Correia e Vale, José Alberto author Tabernero, María Dolores author 2018 [EN]everal classification systems have been proposed to address genomic heterogeneity of glioblastoma multiforme, but they either showed limited prognostic value and/or are difficult to implement in routine diagnostics. Here we propose a prognostic stratification model for these primary tumors based on tumor gene amplification profiles, that might be easily implemented in routine diagnostics, and potentially improve the patients management. Gene amplification profiles were prospectively evaluated in 80 primary glioblastoma multiforme tumors using singlenucleotide polymorphism arrays and the results obtained validated in publicly available data from 267/347 cases. Gene amplification was detected in 45% of patients, and chromosome 7p11.2 including the EGFR gene, was the most frequently amplified chromosomal region - either alone (18%) or in combination with amplification of DNA sequences in other chromosomal regions (10% of cases). Other frequently amplified DNA sequences included regions in chromosomes 12q(10%), 4q12(7%) and 1q32.1(4%). Based on their gene amplification profiles, glioblastomas were subdivided into: i) tumors with no gene amplification (55%); ii) tumors with chromosome 7p/EGFR gene amplification (with or without amplification of other chromosomal regions) (38%); and iii) glioblastoma multiforme with a single (11%) or multiple (6%) amplified DNA sequences in chromosomal regions other than chromosome 7p. González-Tablas, M., Crespo, I., Vital, A. L., Otero, A., Nieto, A. B., Sousa, P., Patino-Alonso, M. C., Corchete, L. A., Tao, H., Rebelo, O., Barbosa, M., & Almeida, M. R. (2018). Prognostic stratification of adult primary glioblastoma multiforme patients based on their tumor gene amplification profiles. Oncotarget, 9(46), 28083–28102. https://doi.org/10.18632/oncotarget.25562 http://hdl.handle.net/10366/146675 10.18632/oncotarget.25562 1949-2553 Classification Gene amplification Glioblastoma Subtypes Survival Prognostic stratification of adult primary glioblastoma multiforme patients based on their tumor gene amplification profiles