2026-09-15T06:13:26Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1507472025-04-30T20:40:53Zcom_10366_149960com_10366_4512com_10366_3823col_10366_149961

Díaz Fernández, David Tatiana Q., Aguiar Martín Herrera, Victoria Isabel Romaní, Aloia Silva, Rui Domingues, Lucília Revuelta Doval, José Luis Jiménez García, Alberto 2022-10-11T08:34:03Z 2022-10-11T08:34:03Z 2019-08 http://hdl.handle.net/10366/150747 10.1016/j.biortech.2019.122054 [EN]This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main Δ9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Ashbya gossypii Microbial lipids Regulation Lignocellulosic hydrolysates Xylose Molasses Glycerol Ashbya gossypii Microbial lipids Regulation Lignocellulosic hydrolysates Xylose Molasses Glycerol Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains info:eu-repo/semantics/article