2026-09-05T21:27:29Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1542512025-04-30T19:41:46Zcom_10366_4028com_10366_3947com_10366_3946com_10366_3823com_10366_154131com_10366_4512col_10366_4029col_10366_154132

Jiménez Gómez, Alejandro Saati Santamaría, Zaki Kostovčik, Martin Rivas González, Raúl Velázquez Pérez, María Encarnación Mateos González, Pedro Francisco Menéndez Gutiérrez, Esther García Fraile, Paula 2024-01-15T13:02:47Z 2024-01-15T13:02:47Z 2020 Jiménez-Gómez, A., Saati-Santamaría, Z., Kostovcik, M., Rivas, R., Velázquez, E., Mateos, P. F., ... & García-Fraile, P. (2020). Selection of the root endophyte Pseudomonas brassicacearum CDVBN10 as plant growth promoter for Brassica napus L. crops. Agronomy, 10(11), 1788. https://doi.org/10.3390/agronomy10111788 http://hdl.handle.net/10366/154251 10.3390/agronomy10111788 2073-4395 [EN]Rapeseed (Brassica napus L.) is an important crop worldwide, due to its multiple uses, such as a human food, animal feed and a bioenergetic crop. Traditionally, its cultivation is based on the use of chemical fertilizers, known to lead to several negative e ects on human health and the environment. Plant growth-promoting bacteria may be used to reduce the need for chemical fertilizers, but e cient bacteria in controlled conditions frequently fail when applied to the fields. Bacterial endophytes, protected from the rhizospheric competitors and extreme environmental conditions, could overcome those problems and successfully promote the crops under field conditions. Here, we present a screening process among rapeseed bacterial endophytes to search for an e cient bacterial strain, which could be developed as an inoculant to biofertilize rapeseed crops. Based on in vitro, in planta, and in silico tests, we selected the strain Pseudomonas brassicacearum CDVBN10 as a promising candidate; this strain produces siderophores, solubilizes P, synthesizes cellulose and promotes plant height in 5 and 15 days-post-inoculation seedlings. The inoculation of strain CDVBN10 in a field trial with no addition of fertilizers showed significant improvements in pod numbers, pod dry weight and shoot dry weight. In addition, metagenome analysis of root endophytic bacterial communities of plants from this field trial indicated no alteration of the plant root bacterial microbiome; considering that the root microbiome plays an important role in plant fitness and development, we suggest this maintenance of the plant and its bacterial microbiome homeostasis as a positive result. Thus, Pseudomonas brassicacearum CDVBN10 seems to be a good biofertilizer to improve canola crops with no addition of chemical fertilizers; this the first study in which a plant growth-promoting (PGP) inoculant specifically designed for rapeseed crops significantly improves this crop’s yields in field conditions. eng MDPI Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Pseudomonas PGPB Bioinoculants Endophytes Bacterial microbiome Culturome Genome sequencing Brassica napus Microbiology Environmental Microbiology 2414 Microbiología microbiología microbiología ambiental Selection of the root endophyte Pseudomonas brassicacearum CDVBN10 as plant growth promoter for Brassica napus L. crops info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://doi.org/10.3390/agronomy10111788 EUROPEAN UNION’S HORIZON 2020, grant number 750795 Agronomy 10 11 1788