2026-09-16T02:39:00Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1542532025-04-30T19:41:47Zcom_10366_4028com_10366_3947com_10366_3946com_10366_3823com_10366_154131com_10366_4512col_10366_4029col_10366_154132

00925njm 22002777a 4500 dc Flores Félix, José David author Velázquez Pérez, María Encarnación author Martínez Molina, Eustoquio author González Andrés, Fernando author Squartini, Andrea author Rivas González, Raúl author 2021 [EN]The legume nodules are a rich source not only of rhizobia but also of endophytic bacteria exhibiting plant growth-promoting mechanisms with potential as plant biostimulants. In this work we analyzed the genomes of Phyllobacterium endophyticum PEPV15 and Rhizobium laguerreae PEPV16 strains, both isolated from Phaseolus vulgaris nodules. In silico analysis showed that the genomes of these two strains contain genes related to N-acyl-homoserine lactone (AHL) and cellulose biosynthesis, involved in quorum sensing and biofilm formation, which are essential for plant colonization. Several genes involved in plant growth promotion such as those related to phosphate solubilization, indole acetic acid production, siderophore biosynthesis and nitrogen fixation were also located in both genomes. When strains PEPV15 and PEPV16 were inoculated in lettuce and carrot in field assays, we found that both significantly increased the yield of lettuce shoots and carrot roots by more than 20% and 10%, respectively. The results of this work confirmed that the genome mining of genes involved in plant colonization and growth promotion is a good strategy for predicting the potential of bacterial strains as crops inoculants, opening new horizons for the selection of bacterial strains with which to design new, effective bacteria-based plant biostimulants. Flores-Félix, J. D., Velázquez, E., Martínez-Molina, E., González-Andrés, F., Squartini, A., & Rivas, R. (2021). Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops. Agronomy, 11(6), 1124. https://doi.org/10.3390/agronomy11061124 http://hdl.handle.net/10366/154253 10.3390/agronomy11061124 2073-4395 Phyllobacterium Rhizobium Carrot Lettuce Bacterial endophytes Plant growth promotion PGPB Genomics Microbial biostimulants Field assays Connecting the lab and the field: Genome analysis of phyllobacterium and rhizobium strains and field performance on two vegetable crops