2026-09-16T04:01:51Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1544302025-04-30T19:41:30Zcom_10366_4028com_10366_3947com_10366_3946com_10366_3823com_10366_154131com_10366_4512col_10366_4029col_10366_154132

Martínez Hidalgo, Pilar Flores Félix, José David Sánchez Juanes, Fernando Rivas González, Raúl Mateos González, Pedro Francisco Santa Regina, Ignacio Peix, Álvaro Martínez Molina, Eustoquio Igual, José M. Velázquez Pérez, María Encarnación 2024-01-19T09:43:26Z 2024-01-19T09:43:26Z 2021 Martínez-Hidalgo, P., Flores-Félix, J. D., Sánchez-Juanes, F., Rivas, R., Mateos, P. F., Santa Regina, I., ... & Velázquez, E. (2021). Identification of canola roots endophytic bacteria and analysis of their potential as biofertilizers for canola crops with special emphasis on sporulating bacteria. Agronomy, 11(9), 1796. http://hdl.handle.net/10366/154430 10.3390/agronomy11091796 2073-4395 [ES] Canola (Brassica napus L. var. oleracea) is the third most common oil-producing crop worldwide after palm and soybean. Canola cultivation requires the use of chemical fertilizers, but the amount required can be reduced by applying plant growth-promoting bacteria (PGPB). Among PGPB, endophytic bacteria have certain advantages as biofertilizers, but canola endophytic bacteria have rarely been studied. In this work, we identified a collection of bacterial endophytes isolated from canola roots using MALDI-TOF MS, a technique that is still rarely used for the identification of such bacteria, and rrs gene sequencing, a methodology that is commonly used to identify canola endophytes. The results demonstrated that some bacterial isolates from canola roots belonged to the genera Bacillus, Neobacillus, Peribacillus (Pe.), and Terribacillus, but most isolates belonged to the genera Paenibacillus (P.) and Pseudomonas (Ps.). Inoculation of these isolates indicated that several of them could efficiently promote canola seedling growth in hydroponic conditions. These results were then confirmed in a microcosm experiment using agricultural soil, which demonstrated that several isolates of Pseudomonas thivervalensis, Paenibacillus amylolyticus, Paenibacillus polymyxa, Paenibacillus sp. (Paenibacillus glucanolyticus/Paenibacillus lautus group), and Peribacillus simplex (previously Bacillus simplex) could efficiently promote canola shoot growth under greenhouse conditions. Among them, the isolates of Paenibacillus and Peribacillus were the most promising biofertilizers for canola crops as they are sporulated rods, which is an advantageous trait when formulating biofertilizers. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional canola Brassica napus rapeseed endophytic bacteria MALDI-TOF MS rrs gene sequencing plant growth promotion Identification of Canola Roots Endophytic Bacteria and Analysis of Their Potential as Biofertilizers for Canola Crops with Special Emphasis on Sporulating Bacteria info:eu-repo/semantics/article