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oai:gredos.usal.es:10366/1548712026-01-14T10:26:22Zcom_10366_4182com_10366_4055com_10366_3946com_10366_3823col_10366_4183

Determination of Hydroxy Polycyclic Aromatic Hydrocarbons in Human Urine Using Automated Microextraction by Packed Sorbent and Gas Chromatography–Mass Spectrometry García García, Samuel Matilla González, Héctor Peña González, Javier Nogal Sánchez, Miguel del Casas Ferreira, Ana María Pérez Pavón, José Luis Polycyclic aromatic hydrocarbon metabolites Urine samples Microextraction by packed sorbent Gas chromatography–mass spectrometry Underivatized analytes [EN]A fast methodology for the determination of monohydroxy polycyclic aromatic hydrocarbons in human urine using a fully automated microextraction by packed sorbent coupled to a gas chromatograph–mass spectrometer is reported. Sample preparation requires simple hydrolysis, centrifugation, filtration, and dilution. The method does not require a derivatization step prior to analysis with gas chromatography and allows the measurement of up to three samples per hour after hydrolysis. Quantitation is carried out by a one-point standard addition allowing the determination of 6 analytes with good limits of detection (10.1–39.6 ng L􀀀1 in water and 0.5–19.4 g L􀀀1 in urine), accuracy (88–110%) and precision (2.1–23.4% in water and 5.1–19.0% in urine) values. This method has been successfully applied to the analysis of six urine samples (three from smoker and three from non-smoker subjects), finding significant differences between both types of samples. Results were similar to those found in the literature for similar samples, which proves the applicability of the methodology. 2024-01-29T10:15:13Z 2024-01-29T10:15:13Z 2022-10-12 info:eu-repo/semantics/article García-García S, Matilla-González H, Peña J, Nogal Sánchez MD, Casas-Ferreira AM, Pérez Pavón JL. Determination of Hydroxy Polycyclic Aromatic Hydrocarbons in Human Urine Using Automated Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry. Int J Environ Res Public Health. 2022 Oct 12;19(20):13089. doi: 10.3390/ijerph192013089. PMID: 36293669; PMCID: PMC9602966. 1660-4601 http://hdl.handle.net/10366/154871 10.3390/ijerph192013089 eng https://doi.org/10.3390/ijerph192013089 SA111P20 FS/1-2021 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional MDPI