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oai:gredos.usal.es:10366/1551052025-04-30T19:41:32Zcom_10366_154956com_10366_4512com_10366_3823com_10366_130154com_10366_4576com_10366_4028com_10366_3947com_10366_3946col_10366_154957col_10366_130155col_10366_4029

00925njm 22002777a 4500 dc Tello Martín, Maria Luisa author Lavega, Rebeca author Pérez, Margarita author Pérez-Pulido, Antonio J. author Carrasco Carrasco, Jaime author Thon, Michael Ronald author Pérez Benito, Ernesto author 2022 [EN]The cultivation of edible mushroom is an emerging sector with a potential yet to be discovered. Unlike plants, it is a less developed agriculture where many studies are lacking to optimize the cultivation. In this work we have employed high-throughput techniques by next generation sequencing to screen the microbial structure of casing soil employed in mushroom cultivation (Agaricus bisporus) while sequencing V3-V4 of the 16S rRNA gene for bacteria and the ITS2 region of rRNA for. In addition, the microbiota dynamics and evolution (bacterial and fungal communities) in peat-based casing along the process of incubation of A. bisporus have been studied, while comparing the efect of fungicide treatment (chlorothalonil and metrafenone). Statistically signifcant changes in populations of bacteria and fungi were observed. Microbial composition difered signifcantly based on incubation day, changing radically from the original communities in the raw material to a specifc microbial composition driven by the A. bisporus mycelium growth. Chlorothalonil treatment seems to delay casing colonization by A. bisporus. Proteobacteria and Bacteroidota appeared as the most dominant bacterial phyla. We observed a great change in the structure of the bacteria popula‑ tions between day 0 and the following days. Fungi populations changed more gradually, with A. bisporus displacing the rest of the species as the cultivation cycle progresses. A better understanding of the microbial communities in the casing will hopefully allow us to increase the biological efciency of the crop. Tello Martín, M.L., Lavega, R., Carrasco Carrasco, J., Pérez, M., Pérez-Pulido, A.J., Thon, M. and Benito Pérez, E. (2022). Influence of Agaricus bisporus establishment and fungicidal treatments on casing soil metataxonomy during mushroom cultivation. BMC Genomics, 23, 1-12. https://doi.org/10.1186/s12864-022-08638-x. http://hdl.handle.net/10366/155105 10.1186/s12864-022-08638-x 1471-2164 Mushroom Metataxonomy Microbiota Agaricus QIIME2 16S ITS Influence of Agaricus bisporus establishment and fungicidal treatments on casing soil metataxonomy during mushroom cultivation