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oai:gredos.usal.es:10366/1551402025-04-30T19:25:50Zcom_10366_3966com_10366_3947com_10366_3946com_10366_3823col_10366_3967

00925njm 22002777a 4500 dc Sanz Andreu, Luis author Montero, Manuel author Grondona, Isabel author Vizcaíno, Juan Antonio author Llobell, Antonio author Hermosa Prieto, María Rosa author Monte Vázquez, Enrique author 2004-10 [EN]Trichoderma is known for being the most frequently used biocontrol agent in agriculture. A fundamental part of the Trichoderma antifungal system relies on a series of genes coding for a variety of extracellular lytic enzymes. Characterization of the polymorphism between five putative isoenzymatic activities [beta-1,3-glucanase (EC 3.2.1.39, EC 3.2.1.58), beta-1,6-glucanase (EC 3.2.1.75), cellulase (EC 3.2.1.4; EC 3.2.1.21, EC 3.2.1.91), chitinase (EC 3.2.1.30, EC 3.2.1.52), protease (EC 3.4.11; EC 3.4.13-19; EC 3.4.21-24, EC 3.4.99)] was carried out using 18 strains from three sections of Trichoderma. Of these, seven strains were from T. sect. Pachybasium, nine from T. sect. Trichoderma and two from T. sect. Longibrachiatum. Thirty-seven different alleles in total were identified: 13 for beta-1,3-glucanase, four for beta-1,6-glucanase, three for cellulase, eight for chitinase and nine for protease activity. A dendrogram (constructed by the unweighted pair group method with arithmetic averages) based on isoenzymatic data separated the 18 strains into three main enzymatic groups: T. harzianum, T. atroviride/T. viride/T. koningii and T. asperellum/T. hamatum/T. longibrachiatum. Isoenzymatic groupings obtained from biocontrol strains are discussed in relation to their phylogenetic location, based on their sequence of internal transcribed spacer 1 in ribosomal DNA and their antifungal activities. Sanz, L., Montero, M., Grondona, I., Vizcaíno, J. A., Llobell, A., Hermosa, R., & Monte, E. (2004). Cell wall-degrading isoenzyme profiles of Trichoderma biocontrol strains show correlation with rDNA taxonomic species. Current genetics, 46, 277-286. doi: 10.1007/s00294-004-0532-6. 0172-8083 http://hdl.handle.net/10366/155140 10.1007/s00294-004-0532-6 15480677 1432-0983 Trichoderma Biocontrol agent Lytic enzymes Genetic diversity Cell wall-degrading isoenzyme profiles of Trichoderma biocontrol strains show correlation with rDNA taxonomic species