2026-09-14T20:23:36Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1551542025-04-30T19:25:44Zcom_10366_3966com_10366_3947com_10366_3946com_10366_3823col_10366_3967

Nucleoredoxin downregulation reduces β-Catenin levels and shifts hematopoietic differentiation towards myeloid lineage in vitro Pérez Fernández, Alejandro López Ruano, Guillermo Prieto Bermejo, Rodrigo Sánchez Bernal, María Carmen Sánchez Yagüe, Jesús Hernández Hernández, Ángel Nucleoredoxin β-catenin Hematopoietic differentiation [EN]The importance of dissecting signaling pathways governing cell differentiation is based on their relevance not only for understanding basic biological phenomena but also for better comprehending the underlying mechanisms of pathologic alterations such as cancer. A paradigm of cell differentiation processes is hematopoiesis, where a single stem cell gives rise to multiple, fully differentiated, cell lineages. Nucleoredoxin (Nrx), a member of the thioredoxin family, is an important redox-sensitive modulator of Wnt/ -catenin signaling, a key pathway for the control of hematopoiesis. In this work, the relevance of Nrx for the differentiation of mouse hematopoietic progenitor cells has been analyzed in vitro. Nrx silencing leads to a dramatic reduction in the size of the Lin􀀀 and LSK progenitor populations. Moreover, there is also a remarkable decrease in CD3+ cells and an enhancement in the percentage of CD11b+Gr1􀀀 myeloid cells. This myeloid bias would agree with the inhibition of the Wnt/ -catenin pathway. Interestingly, a reduction in -catenin at the protein level was observed upon Nrx silencing. Our results strongly support the importance of Nrx for hematopoietic differentiation, which could be mediated by the regulation of the Wnt/ -catenin pathway. 2024-02-01T11:44:10Z 2024-02-01T11:44:10Z 2021-04-04 info:eu-repo/semantics/article Pérez-Fernández, A., López-Ruano, G., Prieto-Bermejo, R., Sánchez-Bernal, C., Sánchez-Yagüe, J., & Hernández-Hernández, Á. (2021). Nucleoredoxin Downregulation Reduces β-Catenin Levels and Shifts Hematopoietic Differentiation towards Myeloid Lineage In Vitro. BioChem, 1(1), 26-35. https://doi.org/10.3390/biochem1010003 http://hdl.handle.net/10366/155154 10.3390/biochem1010003 2673-6411 eng https://doi.org/10.3390/biochem1010003 This research was funded by the Spanish Ministry of Economy and Competitiveness, grant number BFU2014-56490-R. The salaries of A.P.-F., G.L.-R. and R.P.-B. were co-funded by the Junta de Castilla y León and European Social Fund (Ayudas destinadas a la contratación predoctoral de personal investigador). info:eu-repo/semantics/openAccess MDPI