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oai:gredos.usal.es:10366/1552582025-04-30T19:36:48Zcom_10366_4019com_10366_3947com_10366_3946com_10366_3823col_10366_4020

00925njm 22002777a 4500 dc Martinez-Lopez, Joaquin author Lahuerta, Juan José author Pepin, François author González Díaz, Marcos author Barrio, Santiago author Ayala, Rosa author Puig Morón, Noemí author Montalban, María A author Lourenço Paiva, Bruno author Weng, Li author Jiménez, Cristina author Sopena, María author Moorhead, Martin author Cedena, Teresa author Rapado, Immaculada author Mateos Manteca, María Victoria author Rosiñol, Laura author Oriol, Albert author Blanchard, María J. author Martinez, Rafael author Bladé, Joan author San Miguel Izquierdo, Jesús Fernando author Faham, Malek author García Sanz, Ramón author 2014-05-15 [EN]We assessed the prognostic value of minimal residual disease (MRD) detection in multiple myeloma (MM) patients using a sequencing-based platform in bone marrow samples from 133 MM patients in at least very good partial response (VGPR) after front-line therapy. Deep sequencing was carried out in patients in whom a high-frequency myeloma clone was identified and MRD was assessed using the IGH-VDJH, IGH-DJH, and IGK assays. The results were contrasted with those of multiparametric flow cytometry (MFC) and allele-specific oligonucleotide polymerase chain reaction (ASO-PCR). The applicability of deep sequencing was 91%. Concordance between sequencing and MFC and ASO-PCR was 83% and 85%, respectively. Patients who were MRD(-) by sequencing had a significantly longer time to tumor progression (TTP) (median 80 vs 31 months; P < .0001) and overall survival (median not reached vs 81 months; P = .02), compared with patients who were MRD(+). When stratifying patients by different levels of MRD, the respective TTP medians were: MRD ≥10(-3) 27 months, MRD 10(-3) to 10(-5) 48 months, and MRD <10(-5) 80 months (P = .003 to .0001). Ninety-two percent of VGPR patients were MRD(+). In complete response patients, the TTP remained significantly longer for MRD(-) compared with MRD(+) patients (131 vs 35 months; P = .0009). Martinez-Lopez, J., Lahuerta, J. J., Pepin, F., González, M., Barrio, S., Ayala, R., ... & García-Sanz, R. (2014). Prognostic value of deep sequencing method for minimal residual disease detection in multiple myeloma. Blood, The Journal of the American Society of Hematology, 123(20), 3073-3079. https://doi.org/10.1182/blood-2014-01-550020 http://hdl.handle.net/10366/155258 10.1182/blood-2014-01-550020 24646471 1528-0020 Myeloma Deep sequencing Next Generation Sequencing Minimal Residual Disease Prognostic value of deep sequencing method for minimal residual disease detection in multiple myeloma