2026-09-15T21:29:55Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1568382024-03-22T01:03:10Zcom_10366_156566com_10366_4512com_10366_3823col_10366_156568

Dios Pérez, Inmaculada de González Garcinuño, Álvaro Muñoz Barroso, María Isabel Martín del Valle, Eva María 2024-03-21T08:30:01Z 2024-03-21T08:30:01Z 2024 2099-09-09 0022-3549 http://hdl.handle.net/10366/156838 10.1016/j.xphs.2023.10.017 [EN]In order to reduce the side effects of traditional chemotherapy in the treatment of colorectal cancer (CRC), a newdrug delivery system has been developed in this work, based on exosomes that can host two drugs that act synergistically: farnesol (that stops the cell cycle) and paclitaxel (prevents microtubule system depolymerization). Firstly, exosomes were isolated from different cell cultures (from colorectal cancer and from f ibroblast as example of normal cell line) by different methods and characterized by western blot, TEM and DLS, and results showed that they express classical protein markers such as CD9 and HSP-70 and they showed spherical morphology with sizes from 93 nm to 129 nm depending on the source. These exosomes were loaded with both drugs and its effect was studied in vitro. The efficacy was studied by comparing the viability of cell cultures with a colorectal cancer cell line (HCT-116) and a normal cell line (fibroblast HS-5). Results showed that exosomes present a specific effect with more reduction in cell viability in tumour cultures than healthy ones. In summary, exosomes are presented in this work as a promising strategy for colorectal cancer treatment eng Elsevier Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Colorectal cancer Drug targeting Exosomes Drug delivery systems Cell lines Nanotechnology Cyclodextrins Poorly water-soluble drugs Farnesol Paclitaxel Exosomes Colorectal Neoplasms Drug Delivery Systems Cell Line Nanotechnology 3201.01 Oncología 2412.02 Anticuerpos exosomas sistemas de liberación de medicamentos nanotecnología neoplasias colorrectales línea celular A Synergistic Approach Therapy for Colorectal Cancer Based on Exosomes and Exploitation of Metabolic Pathways info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://doi.org/10.1016/j.xphs.2023.10.017 Journal of Pharmaceutical Sciences 113 4 1038 1046