2026-09-17T03:13:46Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1568382024-03-22T01:03:10Zcom_10366_156566com_10366_4512com_10366_3823col_10366_156568

00925njm 22002777a 4500 dc Dios Pérez, Inmaculada de author González Garcinuño, Álvaro author Muñoz Barroso, María Isabel author Martín del Valle, Eva María author 2024 [EN]In order to reduce the side effects of traditional chemotherapy in the treatment of colorectal cancer (CRC), a newdrug delivery system has been developed in this work, based on exosomes that can host two drugs that act synergistically: farnesol (that stops the cell cycle) and paclitaxel (prevents microtubule system depolymerization). Firstly, exosomes were isolated from different cell cultures (from colorectal cancer and from f ibroblast as example of normal cell line) by different methods and characterized by western blot, TEM and DLS, and results showed that they express classical protein markers such as CD9 and HSP-70 and they showed spherical morphology with sizes from 93 nm to 129 nm depending on the source. These exosomes were loaded with both drugs and its effect was studied in vitro. The efficacy was studied by comparing the viability of cell cultures with a colorectal cancer cell line (HCT-116) and a normal cell line (fibroblast HS-5). Results showed that exosomes present a specific effect with more reduction in cell viability in tumour cultures than healthy ones. In summary, exosomes are presented in this work as a promising strategy for colorectal cancer treatment 0022-3549 http://hdl.handle.net/10366/156838 10.1016/j.xphs.2023.10.017 Colorectal cancer Drug targeting Exosomes Drug delivery systems Cell lines Nanotechnology Cyclodextrins Poorly water-soluble drugs Farnesol Paclitaxel A Synergistic Approach Therapy for Colorectal Cancer Based on Exosomes and Exploitation of Metabolic Pathways