2026-09-17T03:16:38Zhttps://gredos.usal.es/oai/request
oai:gredos.usal.es:10366/1569542025-04-30T20:42:04Zcom_10366_156461com_10366_4512com_10366_3823col_10366_156462
In vivo Expression of a Cicer arietinum β-galactosidase in Potato Tubers Leads to a Reduction of the Galactan Side-chains in Cell Wall Pectin Martín Sánchez, José Ignacio Dopico, Berta Muñoz, Francisco J. Esteban Gallego, María Rocío Oomen, Ronald J. F. J. Driouich, Azeddine Vincken, Jean-Paul Visser, Richard Labrador, Emilia Cell wall Chickpea β-galactosidase Pectin Potato Transgenic plant We report the generation of Solanum tuberosum transformants expressing Cicer arietinum βIII-Gal. βIII-Gal is a β-galactosidase able to degrade cell wall pectins during cell wall loosening that occurs prior to cell elongation. cDNA corresponding to the gene encoding this protein was identified among several chickpea β-galactosidase cDNAs, and named CanBGal-3. CanBGal-3 cDNA was expressed in potato under the control of the granule-bound starch synthase promoter. Three βIII-Gal transformants with varying levels of expression were chosen for further analysis. The transgenic plants displayed no significant altered phenotype compared to the wild type. However, β-galactanase and β-galactosidase activities were increased in the transgenic tuber cell walls and this affected the potato tuber pectins. A reduction in the galactosyl content of up to 50% compared to the wild type was observed in the most extreme transformant, indicating a reduction of 1,4-β- galactan side-chains, as revealed by analysis with LM5 specific antibodies. Our results confirm the notion that the pectin-degrading activity of chickpea βIII-Gal reported in vitro also occurs in vivo and in other plants, and confirm the involvement of βIII-Gal in the cell wall autolysis process. An increase in the homogalacturonan content of transgenic tuber cell walls was also observed by Fourier transform infrared spectroscopy (FTIR) analysis. 2024-04-03 2024-04-03 2005 2099-09-09 info:eu-repo/semantics/article 0032-0781 http://hdl.handle.net/10366/156954 10.1093/pcp/pci177 1471-9053 eng CT97 222 BOS2002–01900 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional