2026-09-16T17:27:20Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1571752025-04-30T20:42:03Zcom_10366_156461com_10366_4512com_10366_3823col_10366_156462

Muñoz, Francisco J. Labrador, Emilia Dopico, Berta 2024-04-08T08:27:46Z 2024-04-08T08:27:46Z 1998 0167-4412 http://hdl.handle.net/10366/157175 10.1023/A:1006013718429 A cDNA clone, CanTUB, encoding a putative  tubulin protein was isolated from a cDNA library constructed from 5day old chickpea (Cicer arietinum) epicotyls. Analysis of its deduced amino acid sequence showed all the typical structural motifs of plant  tubulins. Putative sequences for autoregulation and tubulin mRNAstability, GTP and Ca2+/MAPs (microtubuleassociated proteins) binding sites were present. Southern blot analysis of chickpea genomic DNA revealed that there are multiple  tubulin genes. The level of expression of  tubulin genes was correlated with the rate of growth in either seedlings and adult plants. The transcript levels of  tubulin genes were higher in actively elongating tissues such as etiolated epicotyls, roots and stem tissues of adult plants. Brassinolideinduced growth in chickpea epicotyls was accompanied by promotion of the expression of the gene coding for  tubulin. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional Brassinolides Tubulin Cicer arietinum Epicotyls Growth Brassinolides promote the expression of a new Cicer arietinum tubulin gene involved in the epicotyl elongation info:eu-repo/semantics/article