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oai:gredos.usal.es:10366/1573922025-04-30T20:42:01Zcom_10366_156461com_10366_4512com_10366_3823col_10366_156462

Dopico, Berta Nicolás Rodrigo, Gregorio Labrador, Emilia 2024-04-16T08:10:35Z 2024-04-16T08:10:35Z 1990 2099-09-09 0168-9452 http://hdl.handle.net/10366/157392 10.1016/0168-9452(90)90185-Q During the growth of Cicer arietinum epicotyls an increase in specific cell wall beta-glucosidase and beta-galactosidase activities were observed. In contrast, the amount of cell wall proteins extracted with 3 M LiCI decreased between the third and the fourth day of germination, remaining constant up to the seventh day. Partial purification of the cell wall protein extracts from different days by SP­-Sephadex chromatography showed that one fraction increased both in the amount of protein and beta-galactosidase activity throughout the growth of epicotyls. This protein fraction, which is the third beta-galactosidase (betaIII) eluted from a SP-Sephadex chromatography, has been previously characterized by us as the main enzyme involved in the autolytic process. This work was supported by a grant from the Dirección General de Investigación Científica y Técnica, Spain No. PB87-0024. eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess Autolysis Cicer arietinum Epicotyls Beta-galactosidase Glucosidases, growth Changes during epicotyl growth of an autolysis-related β-galactosidase from the cell wall of Cicer arietinum info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PB87-0024 Plant Science 72 1 45 51