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Dopico, Berta Nicolás Rodrigo, Gregorio Labrador, Emilia 2024-04-16T08:10:51Z 2024-04-16T08:10:51Z 1989 2099-09-09 0031-9317 http://hdl.handle.net/10366/157393 10.1111/j.1399-3054.1989.tb05610.x 1399-3054 Two protein fractions with activity as a-galactosidase (EC 3.2.1.22) and a-arabinosi­dase (EC 3.2.1.55), respectively, were identified in the proteins of cell wall of Cicer arietinum L. cv. Castellana extracted with 3 M LiCI. These fractions were partially purified by gel filtration chromatography (Bio Gel P-150), increasing the specific arabinosidase activity 57-fold and the a-galactosidase activity 6-fold. Other protein fractions with glucosidase (EC 3.2.1.21) and glucanase (EC 3.2.1.6) activity also appeared. According to earlier authors, a-arabinosidases and a-galactosidases are related to alterations in linkages occurring in cell walls, since the enzymes are able to hydrolyze isolated wall polymers. However, our preparations hydrolyze intact cell walls only to a very limited extent, such that their participation in the autolytic processes of cell walls can be ruled out. This work was supported by a grant from Comisión Asesora de Investigación Científica y Técnica, Spain no 0395-84. eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/embargoedAccess a-arabinosidase Cell wall Cicer arietinum Epicotyls a-galactosidase Glucanase Partial purification of cell wall α‐galactosidases and α‐arabinosidases from Cicer arietinum epicotyls. Relationship with cell wall autolytic processes info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0395-84 Physiologia Plantarum 75 4 465 468