2026-09-17T03:16:06Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1580862025-04-30T19:23:15Zcom_10366_3948com_10366_3947com_10366_3946com_10366_3823col_10366_3949

Variations of neuronal nitric oxide synthase after hypophysectomy suggest a neuroprotective effect of nitric oxide in response to damage of the neurons of the hypothalamic supraoptic nucleus in the rat Riesco-López, José M. Carretero González, José Vázquez, R. Juanes Méndez, Juan Antonio Blanco, E. Rubio, M. Asensio Gómez, Manuel Riesco-López, José M. nNOS Nitric oxide Hypophysectomy In situ hybridization Immunohistochemistry Optical densitometry nNOS mRNA [EN]The hypothalamic-neurohypophyseal system offers an ideal model to study the processes of nerve degeneration/regeneration after lesions caused by cutting the hypophyseal stalk, or hypophysectomy. Additionally, the magnocellular neurons comprising it contain nitric oxide, and its enzyme, neural nitric oxide synthase, shows important variations in functional alterations of the hypothalamic-neurohypophyseal system. Currently, there is still some controversy as regards whether nitric oxide exerts neurodegenerative or neuroprotective effects in the nervous system. Here we performed a quantitative and qualitative study of the variations in the neuronal activity of nitric oxide synthase, using immunohistochemistry, and of its mRNA, by in situ hybridization, in the magnocellular neurons of the supraoptic nucleus of the hypothalamus of hypophysectomised rats with survival periods of 10-30 days. Our morphological and morphometric results and those pertaining to neuronal quantification and optical density point to a neuroprotective effect of nitric oxide on magnocellular neurons during the regenerative process of the supraoptic nucleus that occurs after hypophysectomy 2024-05-29T07:17:04Z 2024-05-29T07:17:04Z 2008 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 1136-4890 http://hdl.handle.net/10366/158086 eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess https://gredos.usal.es/bitstream/10366/158086/5/AAHH_Variations%20of%20neuronal%20nitric%20oxide.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ Gredos. Repositorio Documental de la Universidad de Salamanca http://hdl.handle.net/10366/158086