2026-09-16T02:40:58Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1581092025-04-30T19:23:14Zcom_10366_3948com_10366_3947com_10366_3946com_10366_3823col_10366_3949

00925njm 22002777a 4500 dc Vázquez, R. author Blanco Barco, Enrique José author Sánchez, F. author Juanes Méndez, Juan Antonio author Rubio, M. author Santos, M. author Vázquez, G. author Hernández, E. author Riesco-López, José M. author Carretero González, José author 2001 [EN]To analyze whether the reorganization of the rat median eminence after hypophysectomy might be related to changes in glial fibrillary acidic protein (GFAP)- and cellular proliferation, the distribution of cells immunoreactive for GFAP and the proliferation rate of such cells were analyzed at 20, 40 and 60 days posthypophysectomy. For this study, four rostro-caudal regions of the median eminence were differentiated: the retrochiasmatic, preinfundibular, infundibular and postinfundibular regions. In each of these regions, three layers were studied: the ependymal, the internal and the external. At 20 and 40 days after hypophysectomy, significant increases in cellular proliferation affecting all three layers studied in the preinfundibular and infundibular regions were found. At the same time points, increases in GFAP expression were also observed. However, after 60 days, GFAP and proliferative cellular nuclear antigen (PCNA) expression decreased. Although variations of PCNA and GFAP levels were evident, no colocalisation of PCNA and GFAP was found in the cells of the median eminence in untreated or hypophysectomized rats when sections were analyzed by double immunohistochemical staining. Our results suggest that reorganization of median eminence involves alterations (or modulation) of GFAPimmunoreactive cells together with a proliferation of cells that are not GFAP-immunoreactive. This study also demonstrates that this reorganization is completed within the first two months after hypophysectomy. 0213-3911 http://hdl.handle.net/10366/158109 Median eminence GFAP-cells Cellular proliferation Hypophysectomy lmmunohistochemistry Characterization of GFAP expression and cell proliferation in the rat median eminence following hypophysectomy