2026-08-25T17:44:57Zhttps://gredos.usal.es/oai/request

oai:gredos.usal.es:10366/1590182025-04-30T19:40:50Zcom_10366_4028com_10366_3947com_10366_3946com_10366_3823col_10366_4029

González de Buitrago Arriero, José Manuel Ferreira, Laura Isidoro García, María Sanz Lozano, Catalina Sofía Lorente Toledano, Félix Dávila González, Ignacio Jesús 2024-07-16T11:19:38Z 2024-07-16T11:19:38Z 2007-10 González-Buitrago JM, Ferreira L, Isidoro-García M, Sanz C, Lorente F, Dávila I. Proteomic approaches for identifying new allergens and diagnosing allergic diseases. Clin Chim Acta. 2007 Oct;385(1-2):21-7. doi: 10.1016/j.cca.2007.07.010. Epub 2007 Jul 19. PMID: 17707361. 0009-8981 http://hdl.handle.net/10366/159018 10.1016/j.cca.2007.07.010 17707361 [EN] Allergic diseases are (IgE)-mediated hypersensitivity reactions affecting more than 25% of the world's population. Proteomic technologies have been increasingly used in the field of allergy and include the use of protein microarrays and two-dimensional gel electrophoresis coupled with immunoblotting. The literature relevant to proteomic approaches to allergic diseases was searched using MEDLINE database. We reviewed proteomics approaches and applications, focusing specifically on two-dimensional immunoblotting techniques and allergen microarrays. The results obtained show that proteomic approaches using two-dimensional immunoblotting appear to be a powerful strategy for the identification of allergenic proteins. Likewise, the use of allergen microarrays allows a large number of IgE antibodies to be simultaneously identified. Proteomic approaches are only beginning to be applied to the study of allergy. In the field of in vitro diagnosis, allergen microarrays provide a promising tool not routinely used in the allergy laboratory. In the near future this powerful technique will be used as a standard technique for in vitro diagnosis of allergy. eng info:eu-repo/semantics/openAccess Allergen Allergic diseases Immunoblotting Microarrays Proteomics Proteomic approaches for identifying new allergens and diagnosing allergic diseases. info:eu-repo/semantics/article