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00925njm 22002777a 4500 dc Sayagués Manzano, José María author Corchete Sánchez, Luis Antonio author Corchete Sánchez, Luis Antonio author Gutiérrez Troncoso, María Laura author Alonso Sarasquete, María Eugenia author Abad Hernández, María Mar author Bengoechea Miranda, Oscar author Fermiñán, Encarna author Anduaga, María Fernanda author Carmen Martínez, Sofía del author Iglesias Iglesias, Manuel José author Esteban Velasco, María Carmen author Angoso Clavijo, María author Alcázar Montero, José Antonio author García García, Jacinto author Orfao de Matos Correia e Vale, José Alberto author Muñoz‐Bellvis, Luís author Sayagués Manzano, José María author Gutiérrez, María Laura author Sarasquete, Maria Eugenia author del Mar Abad, María author Bengoechea, Oscar author del Carmen, Sofia author Iglesias, Manuel author Esteban, Carmen author Angoso, María author Alcázar Montero, José Antonio author García, Jacinto author Orfao de Matos Correia e Vale, José Alberto author Muñoz-Bellvis, Luís author 2016 [EN]Metastatic dissemination is the most frequent cause of death of sporadic colorectal cancer (sCRC) patients. Genomic abnormalities which are potentially characteristic of such advanced stages of the disease are complex and so far, they have been poorly described and only partially understood. We evaluated the molecular heterogeneity of sCRC tumors based on simultaneous assessment of the overall GEP of both coding mRNA and non-coding RNA genes in primary sCRC tumor samples from 23 consecutive patients and their paired liver metastases. Liver metastases from the sCRC patients analyzed, systematically showed deregulated transcripts of those genes identified as also deregulated in their paired primary colorectal carcinomas. However, some transcripts were found to be specifically deregulated in liver metastases (vs. non-tumoral colorectal tissues) while expressed at normal levels in their primary tumors, reflecting either an increased genomic instability of metastatic cells or theiradaption to the liver microenvironment. Newly deregulated metastatic transcripts included overexpression of APOA1, HRG, UGT2B4, RBP4 and ADH4 mRNAS and the miR-3180-3p, miR-3197, miR-3178, miR-4793 and miR-4440 miRNAs, together with decreased expression of the IGKV1-39, IGKC, IGKV1-27, FABP4 and MYLK mRNAS and the miR-363, miR-1, miR-143, miR-27b and miR-28-5p miRNAs. Canonical pathways found to be specifically deregulated in liver metastatic samples included multiple genes related with intercellular adhesion and the metastatic processes (e.g., IGF1R, PIK3CA, PTEN and EGFR), endocytosis (e.g., the PDGFRA, SMAD2, ERBB3, PML and FGFR2), and the cell cycle (e.g., SMAD2, CCND2, E2F5 and MYC). Our results also highlighted the activation of genes associated with the TGFβ signaling pathway, -e.g. RHOA, SMAD2, SMAD4, SMAD5, SMAD6, BMPR1A, SMAD7 and MYC-, which thereby emerge as candidate genes to play an important role in CRC tumor metastasis. Sayagués, J. M., Corchete, L. A., Gutiérrez, M. L., Sarasquete, M. E., Del Mar Abad, M., Bengoechea, O., Fermiñán, E., Anduaga, M. F., Del Carmen, S., Iglesias, M., Esteban, C., Angoso, M., Alcazar, J. A., García, J., Orfao, A., y Muñoz-Bellvis, L. (2016). Genomic characterization of liver metastases from colorectal cancer patients. Oncotarget, 7(45), 72908-72922. https://doi.org/10.18632/oncotarget.12140 http://hdl.handle.net/10366/160917 10.18632/oncotarget.12140 1949-2553 GEP Colorectal cancer Genomic characterization of liver metastases from colorectal cancer patients