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oai:gredos.usal.es:10366/1609842025-04-30T19:40:42Zcom_10366_4028com_10366_3947com_10366_3946com_10366_3823col_10366_4029

San Segundo Val, Ignacio Sanz Lozano, Catalina Sofía Constitutive gene Expression analysis Reference gene Normalization qPCR gene Validation reference gene Capítulo de libro [EN] The quantitative Polymerase Chain Reaction is the most used technique for the study of gene expression. To correct putative experimental errors of this technique is necessary normalizing the expression results of the gene of interest with the obtained for reference genes. Here, we describe an example of the process to select reference genes. In this particular case, we select reference genes for expression studies in the peripheral blood mononuclear cells of asthmatic patients. 2024-12-10 2024-12-10 2016 2099-09-09 info:eu-repo/semantics/article San Segundo-Val, I., & Sanz-Lozano, C. S. (2016). Validation of reference genes in mRNA expression analysis applied to the study of asthma. Molecular Genetics of Asthma, 57-69. 978-1-4939-3650-2 978-1-4939-3652-6 1064-3745 http://hdl.handle.net/10366/160984 10.1007/978-1-4939-3652-6_5 1940-6029 eng https://doi.org/10.1007/978-1-4939-3652-6_5 info:eu-repo/semantics/embargoedAccess Humana Press, New York, NY