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2024-12-10T09:50:53Z urn:hdl:10366/160987 Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma García Solaesa, Virginia Sanz Lozano, Catalina Sofía Binding Electrophoretic mobility shift assay EMSA Gel retardation Protein–DNA complex Capítulo de libro [EN] Electrophoretic mobility shift assays (EMSA) are used to characterize interactions between nucleic acids and proteins in native conditions. This is based on the fact that the electrophoretic mobility of a nucleic acid becomes slower when it forms complexes with proteins. There are many different variants and applications of this methodology. In this chapter we describe a detailed EMSA protocol applied to the study of asthma. 2024-12-10T09:50:53Z 2024-12-10T09:50:53Z 2016 2099-09-09 info:eu-repo/semantics/article García-Solaesa, V., & Sanz-Lozano, C. S. (2016). Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma. Molecular Genetics of Asthma, 91-105. 978-1-4939-3650-2 978-1-4939-3652-6 1064-3745 http://hdl.handle.net/10366/160987 10.1007/978-1-4939-3652-6_7 1940-6029 eng https://doi.org/10.1007/978-1-4939-3652-6_7 info:eu-repo/semantics/embargoedAccess Humana Press, New York, NY